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The accurate, rapid, and convenient detection of viable bacteria is an effective method for reducing infections and illness outbreaks. Here, an unsupervised learning\u2013assisted and surface acoustic wave\u2013interdigital transducer\u2010driven nano\u2010lens holography biosensing platform is developed for the ultrasensitive and amplification\u2010free detection of viable bacteria. The monitoring device integrated with the nano\u2010lens effect can achieve the holographic imaging of polystyrene microsphere probes in an ultra\u2010wide field of view (\u223d28.28\u00a0mm<jats:sup>2<\/jats:sup>), with a sensitivity limit of as low as 99\u00a0nm. A lightweight unsupervised learning hologram processing algorithm considerably reduces training time and computing hardware requirements, without requiring datasets with manual labels. By combining phage\u2013mediated viable bacterial DNA extraction and enhanced CRISPR\u2013Cas12a systems, this strategy successfully achieves the ultrasensitive detection of viable <jats:italic>Salmonella<\/jats:italic> in various real samples, demonstrating enhanced accuracy validated with the qPCR benchmark method. This approach has a low cost (\u223d$500) and is rapid (\u223d1\u00a0h) and highly sensitive (\u223d38\u00a0CFU\u00a0mL<jats:sup>\u22121<\/jats:sup>), allowing for the amplification\u2010free detection of viable bacteria and emerging as a powerful tool for food safety inspection and clinical diagnosis.<\/jats:p>","DOI":"10.1002\/advs.202406912","type":"journal-article","created":{"date-parts":[[2024,11,22]],"date-time":"2024-11-22T04:22:33Z","timestamp":1732249353000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Unsupervised Learning\u2010Assisted Acoustic\u2010Driven Nano\u2010Lens Holography for the Ultrasensitive and Amplification\u2010Free Detection of Viable Bacteria"],"prefix":"10.1002","volume":"12","author":[{"given":"Yang","family":"Zhou","sequence":"first","affiliation":[{"name":"State Key Laboratory of Marine Food Processing and Safety Control Dalian Polytechnic University  Dalian Liaoning 116034 China"},{"name":"Institute of Biopharmaceutical and Health Engineering Shenzhen International Graduate School Tsinghua University  Shenzhen Guangdong 518055 China"},{"name":"College of Engineering Huazhong Agricultural University  Wuhan Hubei 430070 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junpeng","family":"Zhao","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology Huazhong Agricultural University  Wuhan Hubei 430070 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junping","family":"Wen","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology Huazhong Agricultural University  Wuhan Hubei 430070 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziyan","family":"Wu","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology Huazhong Agricultural University  Wuhan Hubei 430070 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongzhen","family":"Dong","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Marine Food Processing and Safety Control Dalian Polytechnic University  Dalian Liaoning 116034 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9309-2730","authenticated-orcid":false,"given":"Yiping","family":"Chen","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Marine Food Processing and Safety Control Dalian Polytechnic University  Dalian Liaoning 116034 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,11,22]]},"reference":[{"key":"e_1_2_8_1_1","doi-asserted-by":"publisher","DOI":"10.1038\/s43016-021-00229-5"},{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1039\/D3CS00417A"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.accounts.1c00355"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trac.2024.117736"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41565-022-01175-4"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.1c02494"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202015628"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.202207879"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cej.2024.148845"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2314030121"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccr.2024.215745"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.202212081"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1039\/D2CS00594H"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1039\/D2LC00289B"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1021\/acssensors.0c02481"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.202105645"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nantod.2024.102238"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2012.052"},{"key":"e_1_2_8_19_1","doi-asserted-by":"crossref","first-page":"1589","DOI":"10.1021\/acs.analchem.2c04703","volume":"95","author":"Zhou Y.","year":"2023","journal-title":"Anal. 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