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Current cleansing options are insufficient to disrupt and remove tenacious biofilms; antibiotic treatments, on the other hand, often fall short against these biofilm\u2010embedded bacteria. This study explores an non\u2010antibiotic approach that extends beyond conventional porphyrin\u2010based phototherapy by using blue light (BL) in conjunction with ferric ions (Fe(III)) to disrupt and eradicate biofilms. The dual not only degraded biofilm extracellular polymeric substances (EPS) in mono\u2010species and polymicrobial biofilms by specifically targeting carboxyl\u2010containing polysaccharides within the matrix but also exhibited broad\u2010spectrum antimicrobial activity by affecting key components of the outer membrane and cell wall. Bacteria, such as\n                    <jats:italic>K. pneumoniae<\/jats:italic>\n                    , with compromised EPS after photolysis, demonstrated increased susceptibility to macrophage phagocytosis. Disruption of the polymicrobial biofilm structure also enhanced the bacterial susceptibility to bactericidal drugs. Treating wounds infected by mixed\u2010species biofilm in diabetic mice demonstrated a substantial reduction in bacterial colonization and improved tissue repair. The BL\u2010Fe(III) modality offers a safe, efficient alternative for managing chronic wound infections, making it ideal for repeated, non\u2010invasive use at home, especially in resource\u2010limited areas.\n                  <\/jats:p>","DOI":"10.1002\/advs.202407898","type":"journal-article","created":{"date-parts":[[2024,12,21]],"date-time":"2024-12-21T12:39:42Z","timestamp":1734784782000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Efficient Photolysis of Multidrug\u2010Resistant Polymicrobial Biofilms"],"prefix":"10.1002","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-2686-2164","authenticated-orcid":false,"given":"Yongli","family":"Li","sequence":"first","affiliation":[{"name":"Wellman Center for Photomedicine Massachusetts General Hospital Department of Dermatology Harvard Medical School  50 Blossom Street Boston MA 02114 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Dong","sequence":"additional","affiliation":[{"name":"Wellman Center for Photomedicine Massachusetts General Hospital Department of Dermatology Harvard Medical School  50 Blossom Street Boston MA 02114 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"ZhengKun","family":"Zhang","sequence":"additional","affiliation":[{"name":"Wellman Center for Photomedicine Massachusetts General Hospital Department of Dermatology Harvard Medical School  50 Blossom Street Boston MA 02114 USA"},{"name":"Institute of Precision Medicine the First Affiliated Hospital Sun Yat\u2010sen University  Guangzhou 510080 P. 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China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5456-5283","authenticated-orcid":false,"given":"Mei X.","family":"Wu","sequence":"additional","affiliation":[{"name":"Wellman Center for Photomedicine Massachusetts General Hospital Department of Dermatology Harvard Medical School  50 Blossom Street Boston MA 02114 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,12,21]]},"reference":[{"key":"e_1_2_9_1_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cis346"},{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.4239\/wjd.v6.i1.37"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1806005115"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01481-16"},{"key":"e_1_2_9_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2020.02.056"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature12790"},{"key":"e_1_2_9_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2014.01.014"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcma.2017.07.012"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.1501632"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioflm.2020.100027"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.4137\/MBI.S13914"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41522-023-00427-y"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.addr.2021.114057"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jphotobiol.2020.112109"},{"key":"e_1_2_9_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/advs.201900030"},{"key":"e_1_2_9_12_3","doi-asserted-by":"publisher","DOI":"10.1172\/jci.insight.153079"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jphotobiol.2020.111790"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiz487"},{"key":"e_1_2_9_14_2","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.aba3571"},{"key":"e_1_2_9_14_3","doi-asserted-by":"publisher","DOI":"10.1038\/s42003-021-01956-y"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.02833-22"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-020-0825-4"},{"key":"e_1_2_9_16_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12866-020-02007-y"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1009376"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-397169-2.00003-2"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s43630-021-00014-0"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.17.1.593"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1008848"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.183.3.1047-1057.2001"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm8111935"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms9102074"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1524-475X.2008.00434.x"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0099-2399(06)80681-3"},{"key":"e_1_2_9_27_1","unstructured":"a)US. 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