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Naturally, only early fertilized embryos possess totipotency, and they lose this ability as they develop. The expansion of stem cell differentiation potential has been a hot topic in developmental biology for years, particularly with respect to the generation totipotent\u2010like stem cells. Here, the study describes the establishment of totipotency in embryonic stem cells (ESCs) via the deletion of a single gene,\n                    <jats:italic>Pdzk1<\/jats:italic>\n                    .\n                    <jats:italic>Pdzk1<\/jats:italic>\n                    \u2010knockout (KO) ESCs substantially contribute to the fetus, placenta, and yolk sac in chimera assays but can also self\u2010organize to form standard blastocyst\u2010like structures containing the three lineages efficiently; thus, they exhibit full developmental potential as early blastomeres. Single\u2010cell transcriptome and bulk RNA\u2010seq comprehensive analyses revealed that\n                    <jats:italic>Pdzk1<\/jats:italic>\n                    \u2010KO activates several lineage inducers (\n                    <jats:italic>C1qa<\/jats:italic>\n                    ,\n                    <jats:italic>C1qb<\/jats:italic>\n                    ,\n                    <jats:italic>Fgf5<\/jats:italic>\n                    , and\n                    <jats:italic>Cdx2<\/jats:italic>\n                    ) to break down barriers between embryonic and extraembryonic tissues, making these lineages switch smoothly and resulting in a totipotent\u2010like state. This versatile and scalable system provides a robust experimental model for differentiation potency and cell fate studies.\n                  <\/jats:p>","DOI":"10.1002\/advs.202408852","type":"journal-article","created":{"date-parts":[[2024,12,4]],"date-time":"2024-12-04T09:03:01Z","timestamp":1733302981000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Capture of Totipotency in Mouse Embryonic Stem Cells in the Absence of\n                    <i>Pdzk1<\/i>"],"prefix":"10.1002","volume":"12","author":[{"given":"Wenhao","family":"Zhang","sequence":"first","affiliation":[{"name":"State Key Laboratory of Medicinal Chemical Biology and College of Pharmacy Nankai Animal Resources Center and Reproductive Regulation Institute of Transplantation Medicine Nankai University  Tianjin 300350 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Center for Stem Cell Research School of Life and Science and Technology Tongji University  Shanghai 200092 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haisong","family":"Wang","sequence":"additional","affiliation":[{"name":"Reproductive Medical Center Henan Key Laboratory of Reproduction and Genetics The First Affiliated Hospital of Zhengzhou University  Henan 450052 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shaochen","family":"Nie","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Medicinal Chemical Biology and College of Pharmacy Nankai Animal Resources Center and Reproductive Regulation Institute of Transplantation Medicine Nankai University  Tianjin 300350 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qingshen","family":"Jia","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Medicinal Chemical Biology and College of Pharmacy Nankai Animal Resources Center and Reproductive Regulation Institute of Transplantation Medicine Nankai University  Tianjin 300350 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dan","family":"Ding","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Medicinal Chemical Biology and College of Pharmacy Nankai Animal Resources Center and Reproductive Regulation Institute of Transplantation Medicine Nankai University  Tianjin 300350 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chao","family":"Tong","sequence":"additional","affiliation":[{"name":"National Clinical Research Center for Child Health and Disorders Ministry of Education Key Laboratory of Child Development and Disorders Children\u2019s Hospital of Chongqing Medical University  Chongqing 401122 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao\u2010Ou","family":"Zhang","sequence":"additional","affiliation":[{"name":"Shanghai Key Laboratory of Maternal and Fetal Medicine 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