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Coating blood\u2010contacting surfaces with antithrombogenic endothelial cells is considered an effective strategy for preventing thrombus formation. However, this concept has not yet been successfully implemented in LVADs, as severe cell loss is to be expected, especially on the impeller surface with high prothrombogenic supraphysiological shear stress. This study presents a strategy that exploits the magnetic attraction of the impeller on ECs loaded with iron oxide nanoparticles (IONPs) to minimize shear stress\u2010induced cell detachment from the rotating magnetic impeller while ensuring antithrombogenic EC adhesion, especially as a bridge until they formed their adhesion\u2010promoting matrix. 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This protocol is key for the development of a biohybrid LVAD impeller that can prevent life\u2010threatening thrombosis and hemorrhage in a future clinical application.<\/jats:p>","DOI":"10.1002\/advs.202408976","type":"journal-article","created":{"date-parts":[[2024,12,21]],"date-time":"2024-12-21T02:00:47Z","timestamp":1734746447000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Citrate\u2010Coated Iron Oxide Nanoparticles Facilitate Endothelialization of Left Ventricular Assist Device Impeller for Improved Antithrombogenicity"],"prefix":"10.1002","volume":"12","author":[{"given":"Jasper L.","family":"Haritz","sequence":"first","affiliation":[{"name":"Department of Cardiothoracic Transplantation and Vascular Surgery Hannover Medical School  Carl\u2010Neuberg\u2010Str. 1 30625 Hannover Germany"},{"name":"Lower Saxony Center for Biomedical Engineering Implant Research and Development  Stadtfelddamm 34 30625 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