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Understanding how A\u03b2 overexpression alters the proteome of individual brain cell types is essential but challenging due to the nature of brain tissue, which contains intermingled various cell types. The current methods for cell\u2010type\u2010specific proteomics either require genetic modifications or complex cell isolation, limiting their use. This study introduces a novel method, in situ cell\u2010type\u2010specific proteome analysis using antibody\u2010mediated biotinylation (iCAB), which applies immunohistochemistry with biotin\u2010tyramide to target cell\u2010specific proteins directly in tissue. Applied to 5xFAD mice, iCAB enables us to identify \u22488000 cell\u2010type\u2010specific proteomes with significantly more differentially expressed proteins than traditional bulk proteome methods, pinpointing unique pathways such as mRNA processing, calcium regulation, and phagocytosis for neurons, astrocytes, and microglia, respectively. 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