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Drawing inspiration from cardiac muscle fiber arrangement, a three\u2010dimensional (3D)\u2010printed multi\u2010layered microporous polycaprolactone (PCL) scaffold is created with interlayer angles set at 45\u00b0 to replicate the precise structure of native cardiac tissue. Compared with the control group and 90\u00b0 PCL scaffolds, the 45\u00b0 PCL scaffolds exhibited superior biocompatibility for cell culture and improved hiPSC\u2010CM maturation in calcium handling. RNA sequencing demonstrated that the 45\u00b0 PCL scaffold promotes the mature phenotype in hiPSC\u2010CMs by upregulating ion channel genes. Using the 45\u00b0 PCL scaffold, a multi\u2010cellular EHT is successfully constructed, incorporating human cardiomyocytes, endothelial cells, and mesenchymal stem cells. These complex EHTs significantly enhanced hiPSC\u2010CM engraftment in vivo, attenuated ventricular remodeling, and improved cardiac function in mouse myocardial infarction. 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