{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T19:58:25Z","timestamp":1790279905611,"version":"4.1.0"},"reference-count":41,"publisher":"Wiley","issue":"48","license":[{"start":{"date-parts":[[2024,11,8]],"date-time":"2024-11-08T00:00:00Z","timestamp":1731024000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,11,8]],"date-time":"2024-11-08T00:00:00Z","timestamp":1731024000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2020YFA0908500"],"award-info":[{"award-number":["2020YFA0908500"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22371062"],"award-info":[{"award-number":["22371062"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100017531","name":"Medical and Health Research Project of Zhejiang Province","doi-asserted-by":"publisher","award":["2022KY1053"],"award-info":[{"award-number":["2022KY1053"]}],"id":[{"id":"10.13039\/501100017531","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advanced Science"],"published-print":{"date-parts":[[2024,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The photo\u2010enzyme coupling system (PECS) holds immense potential in \u201cgreen\u201d biomanufacturing, encompassing the realms of pharmaceuticals, fuels, and carbon sequestration. Nevertheless, the intricate nature of enzymes' structures significantly impedes the seamless integration of multiple enzymes in a precise, tandem fashion, with exact control over their distribution, posing a formidable challenge. Herein, it has devised a mesoporous\n                    <jats:italic>csq<\/jats:italic>\n                    \u2010type metal organic framework (Zr\u2010MOF) from\n                    <jats:italic>meso<\/jats:italic>\n                    \u2010tetrakis\u2010(4\u2010((phenyl)ethynyl)benzoate)porphyrin (Por\u2010PTP) and Zr\n                    <jats:sub>6<\/jats:sub>\n                    (\u03bc\n                    <jats:sub>3<\/jats:sub>\n                    \u2010O)\n                    <jats:sub>4<\/jats:sub>\n                    (\u03bc\n                    <jats:sub>3<\/jats:sub>\n                    \u2010OH)\n                    <jats:sub>4<\/jats:sub>\n                    (OH)\n                    <jats:sub>4<\/jats:sub>\n                    (H\n                    <jats:sub>2<\/jats:sub>\n                    O)\n                    <jats:sub>4<\/jats:sub>\n                    ) nodes (Zr\n                    <jats:sub>6<\/jats:sub>\n                    \u00a0clusters), featuring intricate hierarchical hexagonal (5.8\u00a0nm) and triangular (2.9\u00a0nm) channels, enabling the simultaneous encapsulation of Formate dehydrogenase from\n                    <jats:italic>Candida boidinii<\/jats:italic>\n                    (\n                    <jats:italic>Cb<\/jats:italic>\n                    FDH)\u00a0and ferredoxin\u2010NADP+ reductase (FNR) via a spatiotemporally controlled strategy for cofactor\u2010dependent photoenzymatic carbon dioxide (CO\n                    <jats:sub>2<\/jats:sub>\n                    ) conversion. Upon illumination, photoexcited electrons originating from the Zr\u2010MOF frameworks migrate to the adjacent FNR for cofactor NADH regeneration, which is then harnessed by proximal\n                    <jats:italic>Cb<\/jats:italic>\n                    FDH for CO\n                    <jats:sub>2<\/jats:sub>\n                    fixation. Concurrently, the resulting holes are neutralized by AA for system recovery. The results demonstrated the confinement of tandem enzymes within MOF channels significantly enhanced the performance of multi\u2010enzyme cascade pathways as well as augmenting the local NAD\n                    <jats:sup>+<\/jats:sup>\n                    \/NADH, which leading to a further improvement in the efficiency of tandem biocatalytic formic acid generation (55\u00a0m\n                    <jats:sc>m<\/jats:sc>\n                    ) from CO\n                    <jats:sub>2<\/jats:sub>\n                    . Crucially, the photo\u2010enzyme\u2010coupled factories exhibited remarkable stability alongside exceptional recyclability, attributed to the preservation of MOF skeletons.\n                  <\/jats:p>","DOI":"10.1002\/advs.202410024","type":"journal-article","created":{"date-parts":[[2024,11,8]],"date-time":"2024-11-08T06:39:09Z","timestamp":1731047949000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Spatiotemporal Encapsulation of Tandem Enzymes in Hierarchical Metal\u2013Organic Frameworks for Cofactor\u2010Dependent Photoenzymatic CO\n                    <sub>2<\/sub>\n                    Conversion"],"prefix":"10.1002","volume":"11","author":[{"given":"Yan","family":"Li","sequence":"first","affiliation":[{"name":"Key Laboratory of Organosilicon Chemistry and Material Technology Ministry of Education College of Material Chemistry and Chemical Engineering Hangzhou Normal University  Hangzhou 311121 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jieqiong","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Organosilicon Chemistry and Material Technology Ministry of Education College of Material Chemistry and Chemical Engineering Hangzhou Normal University  Hangzhou 311121 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoqian","family":"Shi","sequence":"additional","affiliation":[{"name":"Key Laboratory of Organosilicon Chemistry and Material Technology Ministry of Education College of Material Chemistry and Chemical Engineering Hangzhou Normal University  Hangzhou 311121 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoxuan","family":"Yu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Organosilicon Chemistry and Material Technology Ministry of Education College of Material Chemistry and Chemical Engineering Hangzhou Normal University  Hangzhou 311121 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuangjiang","family":"Yu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Organosilicon Chemistry and Material Technology Ministry of Education College of Material Chemistry and Chemical Engineering Hangzhou Normal University  Hangzhou 311121 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junqiu","family":"Liu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Organosilicon Chemistry and Material Technology Ministry of Education College of Material Chemistry and Chemical Engineering Hangzhou Normal University  Hangzhou 311121 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0943-991X","authenticated-orcid":false,"given":"Hongcheng","family":"Sun","sequence":"additional","affiliation":[{"name":"Key Laboratory of Organosilicon Chemistry and Material Technology Ministry of Education College of Material Chemistry and Chemical Engineering Hangzhou Normal University  Hangzhou 311121 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,11,8]]},"reference":[{"key":"e_1_2_8_1_1","doi-asserted-by":"publisher","DOI":"10.1039\/D1CS01008E"},{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.accounts.1c00580"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/natrevmats.2017.50"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.accounts.9b00380"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aat9777"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.accounts.1c00719"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202400097"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41565-018-0251-7"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1039\/D1CS00392E"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201901981"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja3009902"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202200261"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202215894"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201814419"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1021\/acscatal.5b02850"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.1c13167"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.nanolett.3c02355"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.201705083"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.nanolett.9b04375"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202202457"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1039\/b200393g"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1021\/cr300014x"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1039\/C8CS00829A"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201909880"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrev.9b00685"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.accounts.8b00521"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nantod.2020.100985"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1039\/C4CS00103F"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202206283"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/ange.202408756"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.9b12828"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41557-022-00931-2"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-017-02103-0"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cej.2023.146186"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1021\/bc990045t"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja512973b"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.6b04996"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.6b03673"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1039\/C6SC01438K"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1986412"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.202314443"}],"container-title":["Advanced Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/advs.202410024","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/advs.202410024","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/advs.202410024","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T14:23:26Z","timestamp":1781274206000},"score":1,"resource":{"primary":{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/10.1002\/advs.202410024"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,11,8]]},"references-count":41,"journal-issue":{"issue":"48","published-print":{"date-parts":[[2024,12]]}},"alternative-id":["10.1002\/advs.202410024"],"URL":"https:\/\/doi.org\/10.1002\/advs.202410024","archive":["Portico"],"relation":{},"ISSN":["2198-3844","2198-3844"],"issn-type":[{"value":"2198-3844","type":"print"},{"value":"2198-3844","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,11,8]]},"assertion":[{"value":"2024-08-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-11-08","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"2410024"}}