{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T20:04:33Z","timestamp":1790366673617,"version":"4.1.0"},"reference-count":68,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2024,12,4]],"date-time":"2024-12-04T00:00:00Z","timestamp":1733270400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,12,4]],"date-time":"2024-12-04T00:00:00Z","timestamp":1733270400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2022YFA0806301"],"award-info":[{"award-number":["2022YFA0806301"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007219","name":"Natural Science Foundation of Shanghai Municipality","doi-asserted-by":"publisher","award":["22ZR1431100"],"award-info":[{"award-number":["22ZR1431100"]}],"id":[{"id":"10.13039\/100007219","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005230","name":"Natural Science Foundation of Chongqing Municipality","doi-asserted-by":"publisher","award":["cstc2021jcyj\u2010msxmX0125"],"award-info":[{"award-number":["cstc2021jcyj\u2010msxmX0125"]}],"id":[{"id":"10.13039\/501100005230","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advanced Science"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Ovarian dysfunction is a major factor leading to female infertility. Understanding how to improve or reshape ovarian function has become an important entry point for preventing and treating female infertility caused by ovarian dysfunction. Here, plant\u2010derived compounds are screened for in vitro activity upon ovarian organoids derived from feeder\u2010free female germline stem cells. Tetrahydroxy stilbene glucoside (TSG) is found to promote the development of ovarian organoids. Single nucleus transcriptome sequencing and spatial transcriptome sequencing are used to establish a comprehensive spatiotemporal map to elucidate the role of TSG in ovarian organoid development, encompassing cell types and subtypes, transcription factors, pseudo\u2010time sequence, and cell communication dynamics. This analysis indicates that TSG promotes ovarian organoid development through the vascular endothelial growth factor A\u2010Eph receptor B2 ligand\u2010receptor pair between granulosa cells and oocytes. This study has enhanced the understanding of the mechanisms of ovarian organoid development, establishes a technical platform for screening compounds for treating infertility and related diseases, and lays a foundation for clinically applying plant\u2010derived compounds.<\/jats:p>","DOI":"10.1002\/advs.202410098","type":"journal-article","created":{"date-parts":[[2024,12,4]],"date-time":"2024-12-04T08:51:09Z","timestamp":1733302269000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Spatial Transcriptome and Single Nucleus Transcriptome Sequencing Reveals Tetrahydroxy Stilbene Glucoside Promotes Ovarian Organoids Development Through the Vegfa\u2010Ephb2 Pair"],"prefix":"10.1002","volume":"12","author":[{"given":"Chunlan","family":"Mu","sequence":"first","affiliation":[{"name":"School of Basic Medical Sciences Key Laboratory of Fertility Preservation and Maintenance of Ministry of Education Ningxia Medical University  Yinchuan 750004 China"},{"name":"Key Laboratory for the Genetics of Developmental &amp; Neuropsychiatric Disorders (Ministry of Education) Bio\u2010X Institutes Shanghai Jiao Tong University  Shanghai 200240 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyong","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory for the Genetics of Developmental &amp; Neuropsychiatric Disorders (Ministry of Education) Bio\u2010X Institutes Shanghai Jiao Tong University  Shanghai 200240 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiamei","family":"Yang","sequence":"additional","affiliation":[{"name":"School of Basic Medical Sciences Key Laboratory of Fertility Preservation and Maintenance of Ministry of Education Ningxia Medical University  Yinchuan 750004 China"},{"name":"School of Pharmacology Ningxia Medical University  Yinchuan 750004 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Geng G.","family":"Tian","sequence":"additional","affiliation":[{"name":"School of Agriculture and Biology Shanghai Jiao Tong University  Shanghai 200240 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hepeng","family":"Bai","sequence":"additional","affiliation":[{"name":"Key Laboratory for the Genetics of Developmental &amp; Neuropsychiatric Disorders (Ministry of Education) Bio\u2010X Institutes Shanghai Jiao Tong University  Shanghai 200240 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenhui","family":"Lin","sequence":"additional","affiliation":[{"name":"School of Life Sciences and Biotechnology Joint International Research Laboratory of Metabolic and Developmental Sciences Shanghai Jiao Tong University  Shanghai 200240 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linhui","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Urology, Changhai Hospital Second Military Medical University (Naval Medical University)  Shanghai 200433 China"},{"name":"Shanghai Key Laboratory of Cell Engineering  Shanghai 200433 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6424-9783","authenticated-orcid":false,"given":"Ji","family":"Wu","sequence":"additional","affiliation":[{"name":"School of Basic Medical Sciences Key Laboratory of Fertility Preservation and Maintenance of Ministry of Education Ningxia Medical University  Yinchuan 750004 China"},{"name":"Key Laboratory for the Genetics of Developmental &amp; Neuropsychiatric Disorders (Ministry of Education) Bio\u2010X Institutes Shanghai Jiao Tong University  Shanghai 200240 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,12,4]]},"reference":[{"key":"e_1_2_9_1_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2020.118181"},{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.adf7760"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-33323-8"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1226889"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature20104"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aat1674"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.abe0237"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.15252\/embj.2022110815"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2021.121213"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jare.2021.03.006"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24020924"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.202210458"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.8186"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.psj.2022.102415"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1590\/1984-3143-ar2020-0013"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1155\/2021\/7924400"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms25126456"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2018.10.092"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2008.01.011"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymthe.2017.04.019"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.2c03923"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.2147\/DDDT.S189969"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1155\/2020\/7635291"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2020.113195"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2020.109946"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1093\/rb\/rbac107"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2022.154309"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2120393119"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells11111724"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41477-023-01360-w"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.4402"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00120.2020"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-021-00815-9"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/ctm2.694"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibtech.2020.05.006"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41576-019-0129-z"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/gah247"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/gaw012"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1161\/HYPERTENSIONAHA.123.21748"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2019.11.033"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41390-020-1093-1"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.psj.2024.103478"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1080\/15548627.2021.2005415"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2018.01.015"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.5152\/eurasianjmed.2018.17361"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1071\/RD18508"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom10091281"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11033-023-09115-8"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.23133"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1002\/embj.201386003"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.1000597"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2002-021910"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.1994.77"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1080\/15548627.2020.1851496"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1093\/biolre\/ioaa171"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn1237"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms232415585"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1869"},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-016-1023-z"},{"key":"e_1_2_9_60_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13195-019-0524-x"},{"key":"e_1_2_9_61_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11427-023-2360-3"},{"key":"e_1_2_9_62_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-017-1382-0"},{"key":"e_1_2_9_63_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.4096"},{"key":"e_1_2_9_64_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkz369"},{"key":"e_1_2_9_65_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.4463"},{"key":"e_1_2_9_66_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2015.05.002"},{"key":"e_1_2_9_67_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41551-020-0578-x"},{"key":"e_1_2_9_68_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.xinn.2021.100141"}],"container-title":["Advanced Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/advs.202410098","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/advs.202410098","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/advs.202410098","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T19:50:59Z","timestamp":1790365859000},"score":1,"resource":{"primary":{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/10.1002\/advs.202410098"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,12,4]]},"references-count":68,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2025,1]]}},"alternative-id":["10.1002\/advs.202410098"],"URL":"https:\/\/doi.org\/10.1002\/advs.202410098","archive":["Portico"],"relation":{},"ISSN":["2198-3844","2198-3844"],"issn-type":[{"value":"2198-3844","type":"print"},{"value":"2198-3844","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,12,4]]},"assertion":[{"value":"2024-08-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-12-04","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"2410098"}}