{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T17:34:59Z","timestamp":1789580099657,"version":"build-2803163510"},"reference-count":75,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2024,12,10]],"date-time":"2024-12-10T00:00:00Z","timestamp":1733788800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2021YFF1200800"],"award-info":[{"award-number":["2021YFF1200800"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82171861"],"award-info":[{"award-number":["82171861"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81971782"],"award-info":[{"award-number":["81971782"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82101448"],"award-info":[{"award-number":["82101448"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advanced Science"],"published-print":{"date-parts":[[2025,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Integrated biochemical and biophysical signals regulate embryonic development. Correct neural tube formation is critical for the development of central nervous system. However, the role of microgravity in neurodevelopment and its underlying molecular mechanisms remain unclear. In this study, the effects of stimulated microgravity (SMG) on the development of human brain organoids are investigated. SMG impairs N\u2010cadherin\u2010based adherens junction formation, leading to neural tube defects associated with dysregulated self\u2010renewal capacity and neuroepithelial disorganization in human brain organoids. Bulk gene expression analyses reveal that SMG alters Hippo and BMP signaling in brain organoids. The neuropathological deficits in SMG\u2010treated organoids can be rescued by regulating YAP\/BMP\/ID1 axis. Furthermore, sing\u2010cell RNA sequencing data show that SMG results in perturbations in the number and function of neural stem and progenitor cell subpopulations. One of these subpopulations senses SMG cues and transmits BMP signals to the subpopulation responsible for tube morphogenesis, ultimately affecting the proliferating cell population. Finally, SMG intervention leads to persistent neurologic damage even after returning to normal gravity conditions. Collectively, this study reveals molecular and cellular abnormalities associated with SMG during human brain development, providing opportunities for countermeasures to maintain normal neurodevelopment in space.<\/jats:p>","DOI":"10.1002\/advs.202410188","type":"journal-article","created":{"date-parts":[[2024,12,10]],"date-time":"2024-12-10T14:05:20Z","timestamp":1733839520000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["The Critical Role of YAP\/BMP\/ID1 Axis on Simulated Microgravity\u2010Induced Neural Tube Defects in Human Brain Organoids"],"prefix":"10.1002","volume":"12","author":[{"given":"Di","family":"Guo","sequence":"first","affiliation":[{"name":"Academy of Medical Engineering and Translational Medicine Tianjin University  Tianjin 300072 China"},{"name":"State Key Laboratory of Advanced Medical Materials and Devices  Tianjin 300072 China"},{"name":"Haihe Laboratory of Brain\u2010Computer Interaction and Human\u2010Machine Integration  Tianjin 300072 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Yao","sequence":"additional","affiliation":[{"name":"Academy of Medical Engineering and Translational Medicine Tianjin University  Tianjin 300072 China"},{"name":"State Key Laboratory of Advanced Medical Materials and Devices  Tianjin 300072 China"},{"name":"Haihe Laboratory of Brain\u2010Computer Interaction and Human\u2010Machine Integration  Tianjin 300072 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen\u2010Wei","family":"Shao","sequence":"additional","affiliation":[{"name":"Academy of Medical Engineering and Translational Medicine Tianjin University  Tianjin 300072 China"},{"name":"State Key Laboratory of Advanced Medical Materials and Devices  Tianjin 300072 China"},{"name":"Haihe Laboratory of Brain\u2010Computer Interaction and Human\u2010Machine Integration  Tianjin 300072 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia\u2010Chen","family":"Zuo","sequence":"additional","affiliation":[{"name":"Academy of Medical Engineering and Translational Medicine Tianjin University  Tianjin 300072 China"},{"name":"State Key Laboratory of Advanced Medical Materials and Devices  Tianjin 300072 China"},{"name":"Haihe Laboratory of Brain\u2010Computer Interaction and Human\u2010Machine Integration  Tianjin 300072 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhe\u2010Han","family":"Chang","sequence":"additional","affiliation":[{"name":"Academy of Medical Engineering and Translational Medicine Tianjin University  Tianjin 300072 China"},{"name":"State Key Laboratory of Advanced Medical Materials and Devices  Tianjin 300072 China"},{"name":"Haihe Laboratory of Brain\u2010Computer Interaction and Human\u2010Machine Integration  Tianjin 300072 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian\u2010Xin","family":"Shi","sequence":"additional","affiliation":[{"name":"Academy of Medical Engineering and Translational Medicine Tianjin University  Tianjin 300072 China"},{"name":"State Key Laboratory of Advanced Medical Materials and Devices  Tianjin 300072 China"},{"name":"Haihe Laboratory of Brain\u2010Computer Interaction and Human\u2010Machine Integration  Tianjin 300072 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nan","family":"Hu","sequence":"additional","affiliation":[{"name":"Academy of Medical Engineering and Translational Medicine Tianjin University  Tianjin 300072 China"},{"name":"State Key Laboratory of Advanced Medical Materials and Devices  Tianjin 300072 China"},{"name":"Haihe Laboratory of Brain\u2010Computer Interaction and Human\u2010Machine Integration  Tianjin 300072 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuang\u2010Qing","family":"Bao","sequence":"additional","affiliation":[{"name":"Academy of Medical Engineering and Translational Medicine Tianjin University  Tianjin 300072 China"},{"name":"State Key Laboratory of Advanced Medical Materials and Devices  Tianjin 300072 China"},{"name":"Haihe Laboratory of Brain\u2010Computer Interaction and Human\u2010Machine Integration  Tianjin 300072 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meng\u2010Meng","family":"Chen","sequence":"additional","affiliation":[{"name":"Academy of Medical Engineering and Translational Medicine Tianjin University  Tianjin 300072 China"},{"name":"State Key Laboratory of Advanced Medical Materials and Devices  Tianjin 300072 China"},{"name":"Haihe Laboratory of Brain\u2010Computer Interaction and Human\u2010Machine Integration  Tianjin 300072 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiu","family":"Fan","sequence":"additional","affiliation":[{"name":"Academy of Medical Engineering and Translational Medicine Tianjin University  Tianjin 300072 China"},{"name":"State Key Laboratory of Advanced Medical Materials and Devices  Tianjin 300072 China"},{"name":"Haihe Laboratory of Brain\u2010Computer Interaction and Human\u2010Machine Integration  Tianjin 300072 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7861-8252","authenticated-orcid":false,"given":"Xiao\u2010Hong","family":"Li","sequence":"additional","affiliation":[{"name":"Academy of Medical Engineering and Translational Medicine Tianjin University  Tianjin 300072 China"},{"name":"State Key Laboratory of Advanced Medical Materials and Devices  Tianjin 300072 China"},{"name":"Haihe Laboratory of Brain\u2010Computer Interaction and Human\u2010Machine Integration  Tianjin 300072 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,12,10]]},"reference":[{"key":"e_1_2_9_1_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41580-021-00424-z"},{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2022.05.005"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2592"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-018-0479-2"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature25742"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2006.06.044"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2009.06.016"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn.4394"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/nsr\/nwaa062"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2023.108177"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.6.1975"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1006\/cbir.1999.0371"},{"key":"e_1_2_9_13_1","volume":"4","author":"Dey S. K.","year":"2009","journal-title":"PLoS One"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1177\/1933719109337544"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2007.10.016"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a002899"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-021-04026-9"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202204018"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2024.04.021"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.058602"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/stem.1883"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/stem.3182"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells10102794"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2018.09.014"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2022.02.020"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-43973-x"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature10137"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2015.10.044"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2018.09.021"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2016.02.009"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-018-0658-1"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2017.10.008"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/ctm2.70005"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20191561"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.adf6927"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.31037"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12964-019-0383-x"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41526-024-00386-4"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/35054077"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2012.02.024"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2017.07.014"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.050096"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.aaf2304"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-20-0212"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-23-4034"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/awae150"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2009.09.035"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.0486-14.2014"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-018-0444-0"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom14010065"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.3390\/bioengineering11010011"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2020.11.002"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1136\/annrheumdis-2016-eular.6390"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.3868"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmed.2022.05.013"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2016.12.004"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.4172\/2157-7633.1000289"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2021.09.037"},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2009.12.025"},{"key":"e_1_2_9_60_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aam5887"},{"key":"e_1_2_9_61_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-024-07204-7"},{"key":"e_1_2_9_62_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrn1986"},{"key":"e_1_2_9_63_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.4487-15.2016"},{"key":"e_1_2_9_64_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.130658"},{"key":"e_1_2_9_65_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.121.320464"},{"key":"e_1_2_9_66_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.abh2810"},{"key":"e_1_2_9_67_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.matbio.2013.04.002"},{"key":"e_1_2_9_68_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2012.07.006"},{"key":"e_1_2_9_69_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-42013-y"},{"key":"e_1_2_9_70_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2018.12.015"},{"key":"e_1_2_9_71_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-024-53858-2"},{"key":"e_1_2_9_72_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2017.07.007"},{"key":"e_1_2_9_73_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41526-020-00123-7"},{"key":"e_1_2_9_74_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41420-024-01846-2"},{"key":"e_1_2_9_75_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actaastro.2018.08.044"}],"container-title":["Advanced Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/advs.202410188","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,6]],"date-time":"2025-10-06T09:02:57Z","timestamp":1759741377000},"score":1,"resource":{"primary":{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/10.1002\/advs.202410188"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,12,10]]},"references-count":75,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2025,2]]}},"alternative-id":["10.1002\/advs.202410188"],"URL":"https:\/\/doi.org\/10.1002\/advs.202410188","archive":["Portico"],"relation":{},"ISSN":["2198-3844","2198-3844"],"issn-type":[{"value":"2198-3844","type":"print"},{"value":"2198-3844","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,12,10]]},"assertion":[{"value":"2024-08-24","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-12-10","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"2410188"}}