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The previous research shows\n                    <jats:italic>SYISL<\/jats:italic>\n                    knockout significantly increases muscle fiber numbers and mass in mice, but the mechanism remains unclear. This study confirms that the\n                    <jats:italic>SYISL<\/jats:italic>\n                    gene, maternal gut microbiota, and their interaction significantly affect the number of muscle fibers in mouse embryos through distinct mechanisms, as\n                    <jats:italic>SYISL<\/jats:italic>\n                    knockout alters maternal gut microbiota composition and boosts butyrate levels in embryonic serum. Both fecal microbiota transplantation and butyrate feeding significantly increase muscle fiber numbers in offspring, with butyrate inhibiting histone deacetylases and increasing histone acetylation in embryonic muscle. Combined analysis of RNA\u2010seq between wild\u2010type and\n                    <jats:italic>SYISL<\/jats:italic>\n                    knockout mice with ChIP\u2010seq for H3K9ac and H3K27ac reveals that\n                    <jats:italic>SYISL<\/jats:italic>\n                    and maternal microbiota interaction regulates myogenesis via the butyrate\u2010HDAC\u2010H3K9ac\/H3K27ac pathway. Furthermore, scRNA\u2010seq analysis shows that\n                    <jats:italic>SYISL<\/jats:italic>\n                    knockout alone significantly increases the number and proportion of myogenic cells and their dynamics, independently of regulating histone acetylation levels. Cell communication analysis suggests that this may be due to the downregulation of signaling pathways such as MSTN and TGF\u03b2. Overall, multiple pathways are highlighted through which\n                    <jats:italic>SYISL<\/jats:italic>\n                    influences embryonic muscle development, offering valuable insights for treating muscle diseases and improving livestock production.\n                  <\/jats:p>","DOI":"10.1002\/advs.202410953","type":"journal-article","created":{"date-parts":[[2024,12,16]],"date-time":"2024-12-16T13:51:04Z","timestamp":1734357064000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["<i>SYISL<\/i>\n                    Knockout Promotes Embryonic Muscle Development of Offspring by Modulating Maternal Gut Microbiota and Fetal Myogenic Cell Dynamics"],"prefix":"10.1002","volume":"12","author":[{"given":"Hao","family":"Zuo","sequence":"first","affiliation":[{"name":"Key Laboratory of Swine Genetics and Breeding of the Ministry of Agriculture and Rural Affairs Huazhong Agricultural University  Wuhan 430070 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