{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T14:25:05Z","timestamp":1785767105342,"version":"3.56.0"},"reference-count":66,"publisher":"Wiley","issue":"22","license":[{"start":{"date-parts":[[2025,5,19]],"date-time":"2025-05-19T00:00:00Z","timestamp":1747612800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2021YFC2700700"],"award-info":[{"award-number":["2021YFC2700700"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81830044"],"award-info":[{"award-number":["81830044"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advanced Science"],"published-print":{"date-parts":[[2025,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Maternal pregestational diabetes mellitus (PGDM) can lead to adverse fetal outcomes, including lasting impacts on pancreatic development. However, the specific impacts of maternal PGDM on cellular functions and intercellular communication within the fetal pancreas remain poorly understood. Here, single\u2010nucleus RNA sequencing and spatial transcriptomics (ST) are employed to investigate cellular responses and spatial changes in the fetal pancreas (E16.5 and E18.5) under maternal PGDM conditions. The findings reveal significant cellular heterogeneity among acinar and beta cells, along with pronounced metabolic stress responses. More importantly, decreased insulin secretion is observed and accompanied by the compensatory increase of Pdx1, Nkx6.2, and Mafa, and substantial alterations in cell\u2010cell communication across multiple cell types. ST analysis further highlights enhanced spatial enrichment in cellular niches exposed to maternal PGDM. These findings provide valuable insights into the molecular mechanisms underlying fetal pancreatic response to maternal PGDM and offer a detailed spatiotemporal perspective on these processes.<\/jats:p>","DOI":"10.1002\/advs.202411126","type":"journal-article","created":{"date-parts":[[2025,5,19]],"date-time":"2025-05-19T08:12:23Z","timestamp":1747642343000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Integrating Spatial and Single\u2010Nucleus Transcriptomic Data to Assess the Effects of Intrauterine Hyperglycemia on Fetal Pancreatic Development"],"prefix":"10.1002","volume":"12","author":[{"given":"Yu","family":"Deng","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology Peking University First Hospital  No. 8 Xishiku Street Beijing 100034 China"},{"name":"Beijing Key Laboratory of Maternal Fetal Medicine of Gestational Diabetes Mellitus  Beijing 100034 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuting","family":"Wan","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Peking University First Hospital  No. 8 Xishiku Street Beijing 100034 China"},{"name":"Beijing Key Laboratory of Maternal Fetal Medicine of Gestational Diabetes Mellitus  Beijing 100034 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zan","family":"Yuan","sequence":"additional","affiliation":[{"name":"Annoroad Gene Technology (Beijing) Co., Ltd  Beijing 100176 China"},{"name":"Agricultural Bioinformatics Key Laboratory of Hubei Province Hubei Engineering Technology Research Center of Agricultural Big Data College of Informatics Huazhong Agricultural University  Wuhan 430070 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8954-2102","authenticated-orcid":false,"given":"Huixia","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Peking University First Hospital  No. 8 Xishiku Street Beijing 100034 China"},{"name":"Beijing Key Laboratory of Maternal Fetal Medicine of Gestational Diabetes Mellitus  Beijing 100034 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,5,19]]},"reference":[{"key":"e_1_2_10_1_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41574-022-00734-y"},{"key":"e_1_2_10_1_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.1002926"},{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2022.12.006"},{"key":"e_1_2_10_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-022-04756-4"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.2337\/dc24-S015"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2023.106439"},{"key":"e_1_2_10_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tem.2024.04.007"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.metabol.2019.153963"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.2337\/dc22-1894"},{"key":"e_1_2_10_6_2","doi-asserted-by":"publisher","DOI":"10.2337\/dc22-2226"},{"key":"e_1_2_10_6_3","doi-asserted-by":"publisher","DOI":"10.1007\/s00125-020-05294-2"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1530\/JOE-18-0493"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/429154"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41422-021-00486-w"},{"key":"e_1_2_10_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(24)00827-4"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s42255-023-00876-x"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2020.11.010"},{"key":"e_1_2_10_11_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13073-021-00941-8"},{"key":"e_1_2_10_11_3","doi-asserted-by":"publisher","DOI":"10.1186\/s13073-023-01179-2"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2022.11.009"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-10189-x"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2019.02.005"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2020.108043"},{"key":"e_1_2_10_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2023.05.007"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1064344"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.59912"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2019.05.031"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2022.05.013"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.140756"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2013.06.019"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2024.122514"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.2337\/db23-0212"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.2337\/db16-1305"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2013.05.018"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/cddis.2014.88"},{"key":"e_1_2_10_26_2","doi-asserted-by":"publisher","DOI":"10.1002\/dmrr.2894"},{"key":"e_1_2_10_26_3","doi-asserted-by":"publisher","DOI":"10.1210\/en.2019-00232"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.201700710R"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.2337\/db15-1657"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1097\/FM9.0000000000000193"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2013.12.002"},{"key":"e_1_2_10_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2020.108067"},{"key":"e_1_2_10_30_3","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-017-0694-z"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00125-010-1685-0"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.2337\/diacare.18.5.611"},{"key":"e_1_2_10_32_2","doi-asserted-by":"publisher","DOI":"10.2337\/dc19-0571"},{"key":"e_1_2_10_32_3","doi-asserted-by":"publisher","DOI":"10.2337\/dc17-0514"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/s42255-021-00516-2"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2014.02.001"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2021.109919"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms5639"},{"key":"e_1_2_10_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2014.03.010"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.2337\/db14-0190"},{"key":"e_1_2_10_37_2","doi-asserted-by":"publisher","DOI":"10.1111\/jpi.12351"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.83532"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.xinn.2021.100141"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2022.04.003"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers16050846"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41588-023-01570-0"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-21246-9"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.4463"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1158\/2159-8290.CD-21-0316"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-019-1663-x"},{"key":"e_1_2_10_47_1","volume":"20","author":"Ren F.","year":"2024","journal-title":"Nat. Biotechnol."},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.4402"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-021-01358-2"}],"container-title":["Advanced Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/advs.202411126","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,12]],"date-time":"2025-11-12T07:43:12Z","timestamp":1762933392000},"score":1,"resource":{"primary":{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/10.1002\/advs.202411126"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,5,19]]},"references-count":66,"journal-issue":{"issue":"22","published-print":{"date-parts":[[2025,6]]}},"alternative-id":["10.1002\/advs.202411126"],"URL":"https:\/\/doi.org\/10.1002\/advs.202411126","archive":["Portico"],"relation":{},"ISSN":["2198-3844","2198-3844"],"issn-type":[{"value":"2198-3844","type":"print"},{"value":"2198-3844","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,5,19]]},"assertion":[{"value":"2024-09-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-05-19","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"2411126"}}