{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T17:24:57Z","timestamp":1787505897157,"version":"build-2736575974"},"reference-count":73,"publisher":"Wiley","issue":"13","license":[{"start":{"date-parts":[[2025,2,11]],"date-time":"2025-02-11T00:00:00Z","timestamp":1739232000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advanced Science"],"published-print":{"date-parts":[[2025,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Targeting oxidative phosphorylation (OXPHOS) through inhibiting the electron transport chain (ETC) has shown promising pre\u2010clinical efficacy in cancer therapy. Although aerobic glycolysis is a hallmark of cancer, emerging evidence suggest OXPHOS is frequently enhanced, providing metabolic advantages for cell proliferation, metastasis, and drug resistance in a variety of aggressive cancer types including non\u2010small cell lung cancer (NSCLC), yet the underlying molecular mechanisms remain elusive. Here it is reported that POU\u2010domain containing family protein POU3F3 is translocated into the nuclei of NSCLC cell lines harboring mutant RAS, where it activates transcription of ATP5PF, an essential component of mitochondrial ATP synthase and consequent ATP production, leading to enhanced NSCLC proliferation and migration. Moreover, it is further found out that ERK1 phosphorylates POU3F3 at the S393 site in the cytoplasm and promotes the nuclear translocation of POU3F3 via receptor importin \u03b21 in RAS mutant NSCLC cells. Mechanistically, RNA sequencing analysis combined with chromatin immunoprecipitation (ChIP) assay revealed that POU3F3 binds to the promoter of ATP5PF, leading to enhanced ATP5PF transcription and ATP production. Together, this study uncovers a novel RAS\u2010POU3F3\u2010ATP5PF axis in facilitating NSCLC progression, providing a new perspective on the understanding of molecular mechanisms for NSCLC progression.<\/jats:p>","DOI":"10.1002\/advs.202411503","type":"journal-article","created":{"date-parts":[[2025,2,11]],"date-time":"2025-02-11T09:56:57Z","timestamp":1739267817000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Phosphorylation of POU3F3 Mediated Nuclear Translocation Promotes Proliferation in Non\u2010Small Cell Lung Cancer through Accelerating ATP5PF Transcription and ATP Production"],"prefix":"10.1002","volume":"12","author":[{"given":"Qi\u2010Gang","family":"Zeng","sequence":"first","affiliation":[{"name":"Nanhai hospital of Traditional Chinese Medicine Jinan University  Guangdong 528200 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Le","family":"Li","sequence":"additional","affiliation":[{"name":"Nanhai hospital of Traditional Chinese Medicine Jinan University  Guangdong 528200 China"},{"name":"Department of Medical Biochemistry and Molecular Biology School of Medicine Jinan University  Guangdong 510632 China"},{"name":"Key Laboratory of Viral Pathogenesis &amp; Infection Prevention and Control (Jinan University) Ministry of Education  Guangdong 510632 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Chang","sequence":"additional","affiliation":[{"name":"Nanhai hospital of Traditional Chinese Medicine Jinan University  Guangdong 528200 China"},{"name":"Department of Medical Biochemistry and Molecular Biology School of Medicine Jinan University  Guangdong 510632 China"},{"name":"Key Laboratory of Viral Pathogenesis &amp; Infection Prevention and Control (Jinan University) Ministry of Education  Guangdong 510632 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong","family":"Sun","sequence":"additional","affiliation":[{"name":"Nanhai hospital of Traditional Chinese Medicine Jinan University  Guangdong 528200 China"},{"name":"Department of Medical Biochemistry and Molecular Biology School of Medicine Jinan University  Guangdong 510632 China"},{"name":"Key Laboratory of Viral Pathogenesis &amp; Infection Prevention and Control (Jinan University) Ministry of Education  Guangdong 510632 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Zheng","sequence":"additional","affiliation":[{"name":"Nanhai hospital of Traditional Chinese Medicine Jinan University  Guangdong 528200 China"},{"name":"Department of Medical Biochemistry and Molecular Biology School of Medicine Jinan University  Guangdong 510632 China"},{"name":"Key Laboratory of Viral Pathogenesis &amp; Infection Prevention and Control (Jinan University) Ministry of Education  Guangdong 510632 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ling\u2010Na","family":"Xue","sequence":"additional","affiliation":[{"name":"Nanhai hospital of Traditional Chinese Medicine Jinan University  Guangdong 528200 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chao\u2010Ling","family":"Liu","sequence":"additional","affiliation":[{"name":"Nanhai hospital of Traditional Chinese Medicine Jinan University  Guangdong 528200 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xia\u2010Qing","family":"Li","sequence":"additional","affiliation":[{"name":"Institute of Nephrology and Blood Purification The First Affiliated Hospital Jinan University  Guangdong 510632 China"},{"name":"Nephrology department The Fifth Affiliated Hospital (Heyuan Shenhe People's Hospital) Jinan University  Guangdong 517000 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruo\u2010Tong","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Metabolism, Digestion, and Reproduction Faculty of Medicine Imperial College London  London W12 0NN UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia\u2010Xin","family":"Gu","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry and Molecular Biology School of Medicine Jinan University  Guangdong 510632 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhao\u2010Rong","family":"An","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry and Molecular Biology School of Medicine Jinan University  Guangdong 510632 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao\u2010Tao","family":"Yao","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry and Molecular Biology School of Medicine Jinan University  Guangdong 510632 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan\u2010Yang","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Respiratory Nanjing First Hospital Nanjing Medical University  Jiangsu 210012 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6637-9891","authenticated-orcid":false,"given":"Jun","family":"Fan","sequence":"additional","affiliation":[{"name":"Nanhai hospital of Traditional Chinese Medicine Jinan University  Guangdong 528200 China"},{"name":"Department of Medical Biochemistry and Molecular Biology School of Medicine Jinan University  Guangdong 510632 China"},{"name":"Key Laboratory of Viral Pathogenesis &amp; Infection Prevention and Control (Jinan University) Ministry of Education  Guangdong 510632 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1260-4234","authenticated-orcid":false,"given":"Yong","family":"Dai","sequence":"additional","affiliation":[{"name":"Nanhai hospital of Traditional Chinese Medicine Jinan University  Guangdong 528200 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,2,11]]},"reference":[{"key":"e_1_2_10_1_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41416-023-02394-9"},{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2024.216705"},{"key":"e_1_2_10_2_2","doi-asserted-by":"publisher","DOI":"10.3390\/cancers15010062"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21763"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(23)00444-8"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.35128"},{"key":"e_1_2_10_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2023.115891"},{"key":"e_1_2_10_6_1","first-page":"44","volume":"12","author":"Wenner C. E.","year":"1952","journal-title":"Cancer Res."},{"key":"e_1_2_10_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccr.2006.04.023"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.123.3191.309"},{"key":"e_1_2_10_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2014.04.001"},{"key":"e_1_2_10_7_3","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2016.02.011"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.15252\/embr.202050827"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcancer.2022.02.002"},{"key":"e_1_2_10_9_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22063245"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-19-3682"},{"key":"e_1_2_10_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrc1097"},{"key":"e_1_2_10_11_1","first-page":"6750","volume":"60","author":"Guerrero S.","year":"2000","journal-title":"Cancer Res."},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a031450"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20051194"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/17.15.4426"},{"key":"e_1_2_10_14_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M209799200"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-023-01441-4"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1387\/ijdb.170260RL"},{"key":"e_1_2_10_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajhg.2019.06.007"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/pin.12042"},{"key":"e_1_2_10_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/labinvest.2013.2"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12672-021-00414-9"},{"key":"e_1_2_10_18_2","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2021.643613"},{"key":"e_1_2_10_18_3","doi-asserted-by":"publisher","DOI":"10.2174\/1566523222666220309150722"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12890-020-01218-3"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2014.03.002"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2022.1037341"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202300756"},{"key":"e_1_2_10_22_2","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202403093"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2023.04.029"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.annonc.2021.06.001"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-024-07205-6"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2013.09.033"},{"key":"e_1_2_10_26_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.RA119.007733"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4615-4871-3_4"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers11060810"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.314815.118"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-11366-y"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2014.02.030"},{"key":"e_1_2_10_31_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00401-017-1783-x"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.2492"},{"key":"e_1_2_10_32_2","first-page":"1665","volume":"7","author":"Zhu H. Y.","year":"2017","journal-title":"Am. J. Cancer Res."},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-19-1624"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers12102819"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccr.2012.02.014"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-018-0019-5"},{"key":"e_1_2_10_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2003.11.136"},{"key":"e_1_2_10_36_3","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-15-0491"},{"key":"e_1_2_10_36_4","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-17-3070"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lungcan.2021.07.005"},{"key":"e_1_2_10_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.lungcan.2023.107293"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1097\/JTO.0b013e318223c099"},{"key":"e_1_2_10_38_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2017.3438"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41571-018-0105-0"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41417-021-00326-4"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1172\/jci.insight.167874"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.apsb.2022.10.023"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40170-015-0138-0"},{"key":"e_1_2_10_43_2","doi-asserted-by":"publisher","DOI":"10.1186\/1748-717X-9-207"},{"key":"e_1_2_10_43_3","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.118.249326"},{"key":"e_1_2_10_43_4","doi-asserted-by":"publisher","DOI":"10.1007\/s10637-017-0444-1"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2006.62"},{"key":"e_1_2_10_44_2","volume":"124","author":"Beutner G.","year":"2017","journal-title":"J. Vis. Exp."},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2015.05.009"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0506580102"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1158\/2159-8290.CD-12-0095"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.xinn.2024.100625"}],"container-title":["Advanced Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/advs.202411503","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,6]],"date-time":"2025-10-06T20:45:12Z","timestamp":1759783512000},"score":1,"resource":{"primary":{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/10.1002\/advs.202411503"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,2,11]]},"references-count":73,"journal-issue":{"issue":"13","published-print":{"date-parts":[[2025,4]]}},"alternative-id":["10.1002\/advs.202411503"],"URL":"https:\/\/doi.org\/10.1002\/advs.202411503","archive":["Portico"],"relation":{},"ISSN":["2198-3844","2198-3844"],"issn-type":[{"value":"2198-3844","type":"print"},{"value":"2198-3844","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,2,11]]},"assertion":[{"value":"2024-09-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-02-11","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"2411503"}}