{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T11:11:04Z","timestamp":1780398664875,"version":"3.54.1"},"reference-count":38,"publisher":"Wiley","issue":"20","license":[{"start":{"date-parts":[[2025,4,2]],"date-time":"2025-04-02T00:00:00Z","timestamp":1743552000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,4,2]],"date-time":"2025-04-02T00:00:00Z","timestamp":1743552000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100008666","name":"Fundaci\u00f3 la Marat\u00f3 de TV3","doi-asserted-by":"publisher","award":["202010\u201010"],"award-info":[{"award-number":["202010\u201010"]}],"id":[{"id":"10.13039\/100008666","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advanced Science"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    In late stages of inherited and acquired retinal diseases such as Stargardt disease (STGD) or dry age\u2010related macular degeneration (AMD), loss of retinal pigment epithelia (RPE) cells and subsequently photoreceptors in the macular area result in a dramatic decline of central visual function. Repopulating this area with functional RPE cells may prevent or decline the progression of photoreceptor loss. In the present study, the viability, survival, and integration of human induced pluripotent stem cell (hiPSC)\u2010derived RPE cells (hiPSC\u2010RPE) is assessed generated using clinical\u2010grade protocol and cultured on a clinically relevant scaffold (poly\u2010L\u2010lactide\u2010\n                    <jats:italic>co<\/jats:italic>\n                    \u2010D, L\u2010lactide, PDLLA) after subretinal implantation in immunosuppressed minipigs for up to 6 weeks. It is shown that transplanted hiPSC\u2010RPE cells maintain the RPE cell features such as cell polarity, hexagonal shape, and cell\u2013cell contacts, and interact closely with photoreceptor outer segments without signs of gliosis or neuroinflammation throughout the entire period of examination. In addition, an efficient immunosuppressing strategy with a continuous supply of tacrolimus is applied. Continuous verification and improvement of existing protocols are crucial for its translation to the clinic. The results support the use of hiPSC\u2010RPE on PDLLA scaffold as a cell replacement therapeutic approach for RPE degenerative diseases.\n                  <\/jats:p>","DOI":"10.1002\/advs.202412301","type":"journal-article","created":{"date-parts":[[2025,4,4]],"date-time":"2025-04-04T05:12:14Z","timestamp":1743743534000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Delivery of Human iPSC\u2010Derived RPE Cells in Healthy Minipig Retina Results in Interaction Between Photoreceptors and Transplanted Cells"],"prefix":"10.1002","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-8126-1006","authenticated-orcid":false,"given":"Anna","family":"Mace\u010dkov\u00e1 Brymov\u00e1","sequence":"first","affiliation":[{"name":"Institute of Animal Physiology and Genetics Czech Academy of Sciences  Libechov 27721 Czech Republic"},{"name":"Department of Cell Biology Faculty of Science Charles University  Prague 12800 Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisco Javier","family":"Rodriguez\u2010Jimenez","sequence":"additional","affiliation":[{"name":"Stem Cell Therapies in Neurodegenerative Diseases Lab Centro de Investigaci\u00f3n Pr\u00edncipe Felipe  Valencia 46012 Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annika","family":"Konrad","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology Justus\u2010Liebig\u2010University Giessen  35390 Giessen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaroslav","family":"Nemesh","sequence":"additional","affiliation":[{"name":"Institute of Animal Physiology and Genetics Czech Academy of Sciences  Libechov 27721 Czech Republic"},{"name":"Department of Cell Biology Faculty of Science Charles University  Prague 12800 Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammed Arshad","family":"Thottappali","sequence":"additional","affiliation":[{"name":"Institute of Macromolecular Chemistry Czech Academy of Sciences  Prague 16200 Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana","family":"Artero\u2010Castro","sequence":"additional","affiliation":[{"name":"Stem Cell Therapies in Neurodegenerative Diseases Lab Centro de Investigaci\u00f3n Pr\u00edncipe Felipe  Valencia 46012 Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruslan","family":"Nyshchuk","sequence":"additional","affiliation":[{"name":"Institute of Animal Physiology and Genetics Czech Academy of Sciences  Libechov 27721 Czech Republic"},{"name":"Department of Cell Biology Faculty of Science Charles University  Prague 12800 Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anastasiia","family":"Kolesnikova","sequence":"additional","affiliation":[{"name":"Institute of Animal Physiology and Genetics Czech Academy of Sciences  Libechov 27721 Czech Republic"},{"name":"Department of Cell Biology Faculty of Science Charles University  Prague 12800 Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brigitte","family":"M\u00fcller","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology Justus\u2010Liebig\u2010University Giessen  35390 Giessen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hana","family":"Studenovska","sequence":"additional","affiliation":[{"name":"Institute of Macromolecular Chemistry Czech Academy of Sciences  Prague 16200 Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jana","family":"Juhasova","sequence":"additional","affiliation":[{"name":"Institute of Animal Physiology and Genetics Czech Academy of Sciences  Libechov 27721 Czech 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