{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T11:22:09Z","timestamp":1789730529953,"version":"4.0.1"},"reference-count":61,"publisher":"Wiley","issue":"20","license":[{"start":{"date-parts":[[2025,4,3]],"date-time":"2025-04-03T00:00:00Z","timestamp":1743638400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,4,3]],"date-time":"2025-04-03T00:00:00Z","timestamp":1743638400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2022YFC2705103"],"award-info":[{"award-number":["2022YFC2705103"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82000676"],"award-info":[{"award-number":["82000676"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advanced Science"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Selective drug delivery to podocytes remains a challenge. Aptamers, nucleic acids that bind specific cells, offer a potential solution, though podocyte\u2010targeting aptamers have not yet been developed. Podocytes stimulated with adriamycin, puromycin aminonucleoside, and high glucose are used to screen an single\u2010stranded DNA (ssDNA)\u00a0library (10\u00b9\u2075 sequences). High\u2010throughput sequencing identifies nucleotide sequences, and the aptamer's affinity, stability, cytotoxicity, uptake, biodistribution (especially to podocyte), target protein and ability to deliver siRNA are evaluated. After 11\u201314 rounds of selection, high\u2010affinity pools are identified. Sequencing reveals 23,848 unique sequences, narrowed down to 12 candidates. Aptamer S7 is specifically bound to podocytes, and its truncated version, RLS\u20102, demonstrates superior affinity (50\u201370\u00a0nM) and improved stability with phosphorothioate modifications. RLS\u20102 exhibits no significant cytotoxicity, is internalized by podocytes, and localized to lysosomes. In adriamycin\u2010induced and diabetic nephropathy mice, RLS\u20102 preferentially accumulates within glomeruli. Its specificity to podocyte is verified by colocalization examination and quantitated via flowcytometry. EPB41L5 is identified as a target protein. Aptamer\u2010siRNA chimeras based on RLS\u20102 successfully downregulate gene expression without the need for transfection reagents in vitro. These findings underscore the potential of RLS\u20102 as a promising agent for the development of podocyte\u2010targeted drug delivery systems.<\/jats:p>","DOI":"10.1002\/advs.202412356","type":"journal-article","created":{"date-parts":[[2025,4,4]],"date-time":"2025-04-04T21:43:47Z","timestamp":1743803027000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Screening and Identification of Novel DNA Aptamer for Targeted Delivery to Injured Podocytes in Glomerular Diseases"],"prefix":"10.1002","volume":"12","author":[{"given":"Chao","family":"Zhou","sequence":"first","affiliation":[{"name":"Department of Nephrology Children's Hospital National Clinical Research Center for Child Health Zhejiang University School of Medicine  Hangzhou 310052 China"},{"name":"Liangzhu Laboratory Zhejiang University School of Medicine  Hangzhou 310058 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhaofeng","family":"Luo","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Zhejiang Province for Aptamers and Theranostic Aptamer Selection Center Hangzhou Institute of Medicine (HIM) Chinese Academy of Sciences  Hangzhou Zhejiang 310022 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zheng","family":"Zhang","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Zhejiang Province for Aptamers and Theranostic Aptamer Selection Center Hangzhou Institute of Medicine (HIM) Chinese Academy of Sciences  Hangzhou Zhejiang 310022 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qing","family":"Ye","sequence":"additional","affiliation":[{"name":"Department of Nephrology Children's Hospital National Clinical Research Center for Child Health Zhejiang University School of Medicine  Hangzhou 310052 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongjie","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Nephrology Children's Hospital National Clinical Research Center for Child Health Zhejiang University School of Medicine  Hangzhou 310052 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanyan","family":"Meng","sequence":"additional","affiliation":[{"name":"Department of Nephrology Children's Hospital National Clinical Research Center for Child Health Zhejiang University School of Medicine  Hangzhou 310052 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiayu","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Nephrology Children's Hospital National Clinical Research Center for Child Health Zhejiang University School of Medicine  Hangzhou 310052 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shifan","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Nephrology Children's Hospital National Clinical Research Center for Child Health Zhejiang University School of Medicine  Hangzhou 310052 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lidan","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Nephrology Children's Hospital National Clinical Research Center for Child Health Zhejiang University School of Medicine  Hangzhou 310052 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6076-3806","authenticated-orcid":false,"given":"Jianhua","family":"Mao","sequence":"additional","affiliation":[{"name":"Department of Nephrology Children's Hospital National Clinical Research Center for Child Health Zhejiang University School of Medicine  Hangzhou 310052 China"},{"name":"Liangzhu Laboratory Zhejiang University School of Medicine  Hangzhou 310058 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,4,3]]},"reference":[{"key":"e_1_2_8_1_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra1106556"},{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1159\/000514550"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.kint.2016.01.012"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41572-020-0196-7"},{"key":"e_1_2_8_5_1","first-page":"56","volume":"8","author":"Mathieson P. W.","year":"2011","journal-title":"New Rev. Nephrol."},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/ndt\/gfab016"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41581-023-00702-3"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.molpharmaceut.1c00511"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.53083"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0009463"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/smll.201200733"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0138870"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2017.04.005"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.apsb.2023.01.017"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.V134910"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.V125909"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.V133658"},{"key":"e_1_2_8_18_1","volume":"18","author":"Sotokawauchi A.","year":"2021","journal-title":"Diabetes Vasc. Disease Res."},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.2337\/db16-1281"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.2505"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.2353\/ajpath.2008.070601"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/apha.13844"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.abb7422"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2023.04.042"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.nanolett.3c01235"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.abc8226"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2020111561"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2022.121562"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI99507"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/cei.13119"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clim.2021.108869"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clim.2023.109273"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa2314471"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2021060794"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1089\/hum.2019.127"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.bioconjchem.6b00057"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41581-022-00586-9"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibtech.2021.09.011"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.2174\/0929867325666181008142831"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1021\/acssensors.0c02237"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.0c11878"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.23085"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1093\/nsr\/nwad113"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41565-021-00858-8"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2010.01.036"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1582-4934.2009.00754.x"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.0c08596"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.9b03281"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0099771"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13238-016-0323-0"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2020.09.022"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.1021\/acscentsci.0c00763"},{"key":"e_1_2_8_53_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202105947"},{"key":"e_1_2_8_54_1","doi-asserted-by":"publisher","DOI":"10.2217\/17435889.3.5.703"},{"key":"e_1_2_8_55_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41578-018-0038-3"},{"key":"e_1_2_8_56_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrd.2016.199"},{"key":"e_1_2_8_57_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1617004114"},{"key":"e_1_2_8_58_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2021.108883"},{"key":"e_1_2_8_59_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.kint.2021.05.035"},{"key":"e_1_2_8_60_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jiec.2017.07.025"},{"key":"e_1_2_8_61_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkg595"}],"container-title":["Advanced Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/advs.202412356","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/advs.202412356","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/advs.202412356","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,17]],"date-time":"2026-02-17T10:13:54Z","timestamp":1771323234000},"score":1,"resource":{"primary":{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/10.1002\/advs.202412356"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,4,3]]},"references-count":61,"journal-issue":{"issue":"20","published-print":{"date-parts":[[2025,5]]}},"alternative-id":["10.1002\/advs.202412356"],"URL":"https:\/\/doi.org\/10.1002\/advs.202412356","archive":["Portico"],"relation":{},"ISSN":["2198-3844","2198-3844"],"issn-type":[{"value":"2198-3844","type":"print"},{"value":"2198-3844","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,4,3]]},"assertion":[{"value":"2024-10-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-04-03","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"2412356"}}