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Herein, the broad\u2010spectrum capture and detection capability of the antibody\u2010free electropositive nanoprobe are clarified for bacteria for the first time and an ultrasensitive fluorescent ICA platform is constructed for the simultaneous diagnosis of multiple pathogens. A magnetic multilayer quantum dot nanocomposite with an amino\u2010embedded SiO\n                    <jats:sub>2<\/jats:sub>\n                    shell (MagMQD@Si\n                    <jats:sup>+<\/jats:sup>\n                    ) is designed to enrich bacteria from solutions effectively, offer high luminescence, and reduce background signals on test strips, thus greatly improving the sensitivity and stability of ICA technique for pathogen. The superior performance of the MagMQD@Si\n                    <jats:sup>+<\/jats:sup>\n                    \u2010based ICA through the multiplex detection of three common pathogens is demonstrated, namely,\n                    <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                    ,\n                    <jats:italic>Streptococcus pneumoniae<\/jats:italic>\n                    , and\n                    <jats:italic>Salmonella typhimurium<\/jats:italic>\n                    , showing that this ICA possesses high sensitivity (8\u201340 cells mL\n                    <jats:sup>\u22121<\/jats:sup>\n                    ), good reproducibility (relative standard deviation\n                    <jats:italic>&lt;<\/jats:italic>\n                    5.4%), and high specificity for the three target bacteria. The clinical utility of the proposed method is verified through the detection of 30 real sputum samples from patients with bacterial respiratory infections, revealing that the MagMQD@Si\n                    <jats:sup>+<\/jats:sup>\n                    \u2010based ICA has massive potential as a powerful inspection tool for the rapid, sensitive, and ultrasensitive diagnosis of bacterial infections. [Correction added on 29 January 2025, after first online publication:\n                    <jats:italic>Streptococcus typhi<\/jats:italic>\n                    was corrected to\n                    <jats:italic>Salmonella typhimurium<\/jats:italic>\n                    .]\n                  <\/jats:p>","DOI":"10.1002\/advs.202412421","type":"journal-article","created":{"date-parts":[[2025,1,13]],"date-time":"2025-01-13T14:24:57Z","timestamp":1736778297000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Electropositive Magnetic Fluorescent Nanoprobe\u2010Mediated Immunochromatographic Assay for the Ultrasensitive and Simultaneous Detection of Bacteria"],"prefix":"10.1002","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1396-6571","authenticated-orcid":false,"given":"Jiaxuan","family":"Li","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory Medicine Guangdong Provincial 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