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Herein, the simultaneously emergency treatment and dynamic luminescence severity assessment (SETLSA) strategy is designed for SCI based on cerium (Ce)\u2010doped upconversion antioxidant nanoenzymes (Ce@UCNP\u2010BCH). Ce@UCNP\u2010BCH can not only efficiently eliminate the SCI localized ROS, but dynamically monitor the oxidative state in the SCI repair process using a ratiometric luminescence signal. Moreover, the classic basso mouse scale score and immunofluorescence analysis together exhibit that Ce@UCNP\u2010BCH effectively facilitates the regeneration of spinal cord including myelin sheath, and promotes the functional recovery of SCI mice. Particularly, the study combines snATAC\u2010eq and snRNA\u2010seq to reveal the heterogeneity of spinal cord tissue following Ce@UCNP\u2010BCH treatment. The findings reveal a significant increase in myelinating oligodendrocytes, as well as higher expression of myelination\u2010related genes, and the study also reveals the gene regulatory dynamics of remyelination after treatment. Besides, the ETLSA strategy synergistically boosts ROS consumption through the superoxide dismutase (SOD)\u2010related pathways after SOD\u2010siRNA treatment. In conclusion, this SETLSA strategy with simultaneously blocking and dynamic monitoring oxidative stress has enriched the toolkit for promoting SCI repair.<\/jats:p>","DOI":"10.1002\/advs.202412526","type":"journal-article","created":{"date-parts":[[2025,1,9]],"date-time":"2025-01-09T06:26:11Z","timestamp":1736403971000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Single\u2010Cell Multi\u2010omics Assessment of Spinal Cord Injury Blocking via Cerium\u2010doped Upconversion Antioxidant Nanoenzymes"],"prefix":"10.1002","volume":"12","author":[{"given":"Ke","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Orthopedics The First Affiliated Hospital Jinan University  Guangzhou 510632 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Judun","family":"Zheng","sequence":"additional","affiliation":[{"name":"Molecular Diagnosis and Treatment Center for Infectious Diseases 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