{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T22:41:36Z","timestamp":1792190496354,"version":"4.4.0"},"reference-count":26,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2024,12,24]],"date-time":"2024-12-24T00:00:00Z","timestamp":1734998400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82172693"],"award-info":[{"award-number":["82172693"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advanced Science"],"published-print":{"date-parts":[[2025,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Intrahepatic cholangiocarcinoma (ICC) tumor cells and their interactions with the immune microenvironment, particularly at the leading\u2010edge area, have been underexplored. This study employs single\u2010cell RNA sequencing (scRNA\u2010seq) and spatial transcriptome (ST) analysis on samples from the tumor core, adjacent non\u2010tumorous tissue, and the leading\u2010edge area of nine ICC patients. These findings indicate that tumor cells at the leading\u2010edge area demonstrate enhanced proliferation and are tightly associated with the stroma, including endothelial cells and POSTN+ FAP+ fibroblasts. Notably, CD8+ T cells in this region exhibit a naive phenotype with low cytotoxicity and signs of exhaustion, likely due to compromised antigen presentation by antigen\u2010presenting cells (APCs). The predominant CD8+ T cell subset, mucosal\u2010associated invariant T (MAIT) cells, recruits SPP1+ macrophages within the stroma. This interaction, along with the presence of POSTN+ cancer\u2010associated fibroblasts (CAFs) and endothelial cells, forms a unique \u201ctriad structure\u201d that fosters tumor growth and ICC progression. The research highlights the intricate characteristics and interactions of ICC tumor cells in the leading\u2010edge area, offering insights into potential therapeutic targets for intervention.<\/jats:p>","DOI":"10.1002\/advs.202412740","type":"journal-article","created":{"date-parts":[[2024,12,24]],"date-time":"2024-12-24T03:09:14Z","timestamp":1735009754000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":45,"title":["Single\u2010Cell and Spatial Transcriptomics Delineate the Microstructure and Immune Landscape of Intrahepatic Cholangiocarcinoma in the Leading\u2010Edge Area"],"prefix":"10.1002","volume":"12","author":[{"given":"Li","family":"Zuyin","sequence":"first","affiliation":[{"name":"Department of Hepatobiliary Surgery Peking University Organ Transplantation Institute Peking University People's Hospital  Beijing 100044 China"},{"name":"Beijing Key Surgical Basic Research Laboratory of Liver Cirrhosis and Liver Cancer  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