{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T09:50:07Z","timestamp":1792403407907,"version":"4.4.0"},"reference-count":39,"publisher":"Wiley","issue":"37","license":[{"start":{"date-parts":[[2025,7,18]],"date-time":"2025-07-18T00:00:00Z","timestamp":1752796800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,7,18]],"date-time":"2025-07-18T00:00:00Z","timestamp":1752796800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2023YFE0204900"],"award-info":[{"award-number":["2023YFE0204900"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2023YFC3403100"],"award-info":[{"award-number":["2023YFC3403100"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22175107"],"award-info":[{"award-number":["22175107"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22305140"],"award-info":[{"award-number":["22305140"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["2024T170328"],"award-info":[{"award-number":["2024T170328"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["2024M751099"],"award-info":[{"award-number":["2024M751099"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["GZB20240263"],"award-info":[{"award-number":["GZB20240263"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advanced Science"],"published-print":{"date-parts":[[2025,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>mRNA vaccines have been widely implemented and revolutionized the landscape of oncology and epidemiology. Potent vaccination demands precise control over injection depth to ensure optimal lymphatic drainage, driving the development of non\u2010invasive imaging techniques to visualize the distribution and efficacy of mRNA vaccines. Herein, near\u2010infrared\u2010II (NIR\u2010II) lipid nanoparticles (NIR\u2010II\u2010LNPs) are fabricated for mRNA delivery by integrating a cationic fluorophore amphiphile (IR780\u2010C16) into traditional LNPs, enabling longitudinal, quantitative, and non\u2010invasive trafficking of mRNA vaccines. The in vivo behaviors of mRNA vaccines are characterized with different vaccination protocols in mice and rabbits by NIR\u2010II fluorescence imaging. Results proved that following a 25\u00a0\u00b5L injection of mRNA vaccines into the mouse thigh at a depth of 4\u00a0mm or a 500\u00a0\u00b5L injection volume into a rabbit at a depth of 7\u00a0mm via the intramuscular route, the vaccines rapidly and abundantly accumulated in the inguinal lymph nodes. Flow cytometry and tissue immunofluorescence analyses further validated that optimized vaccination parameters effectively activated antitumor immunity and greatly boosted the therapeutic efficacy in B16\u2010OVA and HPV\u2010associated mouse tumor models. NIR\u2010II\u2010LNPs offer a unique modality for non\u2010invasively monitoring the fate of vaccines, reinforcing its role in preclinical and clinical mRNA therapeutics.<\/jats:p>","DOI":"10.1002\/advs.202413014","type":"journal-article","created":{"date-parts":[[2025,7,19]],"date-time":"2025-07-19T02:39:19Z","timestamp":1752892759000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Potentiating the Efficacy of mRNA Vaccines through NIR\u2010II Imaging\u2010Guided Precise Vaccination"],"prefix":"10.1002","volume":"12","author":[{"given":"Mengfei","family":"Li","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology First Hospital of Jilin University  Changchun 130021 P. R. China"},{"name":"Joint Laboratory of Opto\u2010Functional Theranostics in Medicine and Chemistry The First Hospital of Jilin University  Changchun 130021 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xue","family":"Zheng","sequence":"additional","affiliation":[{"name":"Joint Laboratory of Opto\u2010Functional Theranostics in Medicine and Chemistry The First Hospital of Jilin University  Changchun 130021 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinyang","family":"Yu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Bioorganic Phosphorus Chemistry &amp; Chemical Biology Department of Chemistry Tsinghua University  Beijing 100084 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaolong","family":"Qi","sequence":"additional","affiliation":[{"name":"Key Laboratory of Bioorganic Phosphorus Chemistry &amp; Chemical Biology Department of Chemistry Tsinghua University  Beijing 100084 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2412-6121","authenticated-orcid":false,"given":"Shoujun","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology First Hospital of Jilin University  Changchun 130021 P. R. China"},{"name":"Joint Laboratory of Opto\u2010Functional Theranostics in Medicine and Chemistry The First Hospital of Jilin University  Changchun 130021 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guocan","family":"Yu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Bioorganic Phosphorus Chemistry &amp; Chemical Biology Department of Chemistry Tsinghua University  Beijing 100084 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Songling","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology First Hospital of Jilin University  Changchun 130021 P. R. China"},{"name":"Joint Laboratory of Opto\u2010Functional Theranostics in Medicine and Chemistry The First Hospital of Jilin University  Changchun 130021 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,7,18]]},"reference":[{"key":"e_1_2_8_1_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2021.12.033"},{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41578-021-00358-0"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrd.2017.243"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41571-023-00811-9"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12943-021-01335-5"},{"key":"e_1_2_8_6_1","first-page":"11","volume":"35","author":"Huang P.","year":"2023","journal-title":"Adv. Mater."},{"key":"e_1_2_8_7_1","first-page":"10","volume":"119","author":"Chen J. J.","year":"2022","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41568-021-00346-0"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc.2016.16"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.1997.03540450065037"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.321.7271.1237"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41551-019-0378-3"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41541-024-00900-5"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/EXP.20210008"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2023.1302891"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41557-020-00554-5"},{"key":"e_1_2_8_17_1","first-page":"6","volume":"35","author":"Zhu P. D.","year":"2024","journal-title":"Chin. Chem. Lett."},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.201900035"},{"key":"e_1_2_8_19_1","first-page":"25","volume":"31","author":"Zhu S. J.","year":"2019","journal-title":"Adv. Mater."},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.0c07022"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202007040"},{"key":"e_1_2_8_22_1","first-page":"11","volume":"5","author":"Tian R.","year":"2019","journal-title":"Sci. Adv."},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202102640"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201307346"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.9b12469"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.88815"},{"key":"e_1_2_8_27_1","first-page":"10","volume":"63","author":"Li Q. Q.","year":"2024","journal-title":"Angew. Chem. Int. Edit."},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202107877"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202111767"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202009986"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa2116620"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(21)00396-0"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2016.09.031"},{"key":"e_1_2_8_34_1","first-page":"20","volume":"13","author":"Liu X.","year":"2021","journal-title":"Sci. Transl. Med."},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202108342"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.adn4955"},{"key":"e_1_2_8_37_1","first-page":"18","volume":"15","author":"Silva J. R.","year":"2023","journal-title":"Sci. Transl. Med."},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13046-021-01841-w"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-07943-y"}],"container-title":["Advanced Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/advs.202413014","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/advs.202413014","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/advs.202413014","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T17:07:02Z","timestamp":1783012022000},"score":1,"resource":{"primary":{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/10.1002\/advs.202413014"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,18]]},"references-count":39,"journal-issue":{"issue":"37","published-print":{"date-parts":[[2025,10]]}},"alternative-id":["10.1002\/advs.202413014"],"URL":"https:\/\/doi.org\/10.1002\/advs.202413014","archive":["Portico"],"relation":{},"ISSN":["2198-3844","2198-3844"],"issn-type":[{"value":"2198-3844","type":"print"},{"value":"2198-3844","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7,18]]},"assertion":[{"value":"2024-10-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-07-18","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e13014"}}