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Herein, the study reports an engineered bacterial\u2010based delivery system that can simultaneously promote CTLs infiltration and control PD\u2010L1 binding protein (PD\u2010L1 trap) release on demand at tumor site. The drug release button of this tumor targeting system is the specific temperature, which is accomplished by dual\u2010modified melanin nanoparticles with photothermal conversion capacity on the engineered bacterial. These dual\u2010modified nanoparticles can form in situ reservoir of heat supplier and antitumor immunity activator once arriving at tumor microenvironment (TME). Importantly, the study establishes the personalized administration regimen according to TME changes, and perform local laser irradiation to trigger PD\u2010L1 trap production only in TME when infiltrated CTLs reach the highest level. 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