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However, existing SnCs\u2010scavenging drugs \u201csenolytics\u201d may lead to the exhaustion of stem and progenitor cells, impairing chondrocyte proliferation and cartilage regeneration. Here, ADAM19, a kind of endopeptidases from the ADAM (a disintegrin and metalloproteinase) family, is identified as a novel target for senescent chondrocyte rejuvenation. ADAM19 is elevated in senescent chondrocytes in both mice and human osteoarthritic joints, as well as in cellular senescence model in vitro. ADAM19 knockdown not only significantly attenuated senescent phenotype of chondrocytes, but also promoted cell proliferation and extracellular matrix synthesis. RNA sequencing revealed ADAM19 may regulate chondrocyte senescence mainly through the PI3K\/AKT signal axis. In addition, a senescence\u2010targeting small interfering RNA (siRNA) delivery system is developed for in vivo delivery of therapeutic siRNA. The complex selectively released ADAM19 siRNA in SnCs and performed high silencing effect on target gene. Furthermore, intra\u2010articular (IA) injection of the complex once every two weeks in OA mice effectively reduced SnCs accumulation and promoted hyaline cartilage regeneration. This study provides a promising strategy for the development of regenerative RNA interference therapy.<\/jats:p>","DOI":"10.1002\/advs.202414419","type":"journal-article","created":{"date-parts":[[2025,2,11]],"date-time":"2025-02-11T02:19:30Z","timestamp":1739240370000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Rejuvenating Hyaline Cartilage with Senescence\u2010Targeting Si\u2010ADAM19 Delivery for Osteoarthritis Therapy"],"prefix":"10.1002","volume":"12","author":[{"given":"Jiasheng","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Sports Medicine of the Second Affiliated Hospital and Liangzhu Laboratory Zhejiang University School of Medicine  Hangzhou 310058 China"},{"name":"Dr. Li Dak Sum &amp; Yip Yio Chin Center for Stem Cells and Regenerative Medicine Zhejiang University School of Medicine  Hangzhou 310058 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Sports Medicine of the Second Affiliated Hospital and Liangzhu Laboratory Zhejiang University School of Medicine  Hangzhou 310058 China"},{"name":"Dr. Li Dak Sum &amp; Yip Yio Chin Center for Stem Cells and Regenerative Medicine Zhejiang University School of Medicine  Hangzhou 310058 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongmei","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Sports Medicine of the Second Affiliated Hospital and Liangzhu Laboratory Zhejiang University School of Medicine  Hangzhou 310058 China"},{"name":"Dr. Li Dak Sum &amp; Yip Yio Chin Center for Stem Cells and Regenerative Medicine Zhejiang University School of Medicine  Hangzhou 310058 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junzhi","family":"Yi","sequence":"additional","affiliation":[{"name":"Department of Sports Medicine of the Second Affiliated Hospital and Liangzhu Laboratory Zhejiang University School of Medicine  Hangzhou 310058 China"},{"name":"Dr. Li Dak Sum &amp; Yip Yio Chin Center for Stem Cells and Regenerative Medicine Zhejiang University School of Medicine  Hangzhou 310058 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qi","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Sports Medicine of the Second Affiliated Hospital and Liangzhu Laboratory Zhejiang University School of Medicine  Hangzhou 310058 China"},{"name":"Dr. Li Dak Sum &amp; Yip Yio Chin Center for Stem Cells and Regenerative Medicine Zhejiang University School of Medicine  Hangzhou 310058 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiajie","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Sports Medicine of the Second Affiliated Hospital and Liangzhu Laboratory Zhejiang University School of Medicine  Hangzhou 310058 China"},{"name":"Dr. Li Dak Sum &amp; Yip Yio Chin Center for Stem Cells and Regenerative Medicine Zhejiang University School of Medicine  Hangzhou 310058 China"},{"name":"China Orthopedic Regenerative Medicine Group (CORMed)  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0627-2536","authenticated-orcid":false,"given":"Hongwei","family":"Ouyang","sequence":"additional","affiliation":[{"name":"Department of Sports Medicine of the Second Affiliated Hospital and Liangzhu Laboratory Zhejiang University School of Medicine  Hangzhou 310058 China"},{"name":"Dr. Li Dak Sum &amp; Yip Yio Chin Center for Stem Cells and Regenerative Medicine Zhejiang University School of Medicine  Hangzhou 310058 China"},{"name":"Zhejiang University\u2010University of Edinburgh Institute Zhejiang University School of Medicine  Haining 310058 China"},{"name":"China Orthopedic Regenerative Medicine Group (CORMed)  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,2,10]]},"reference":[{"key":"e_1_2_9_1_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(19)30417-9"},{"key":"e_1_2_9_2_1","first-page":"5","volume":"28","author":"Johnson V. 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