{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T01:37:18Z","timestamp":1792201038364,"version":"4.4.0"},"reference-count":37,"publisher":"Wiley","issue":"19","license":[{"start":{"date-parts":[[2025,3,21]],"date-time":"2025-03-21T00:00:00Z","timestamp":1742515200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22404178"],"award-info":[{"award-number":["22404178"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004735","name":"Natural Science Foundation of Hunan Province","doi-asserted-by":"publisher","award":["2023JJ40711"],"award-info":[{"award-number":["2023JJ40711"]}],"id":[{"id":"10.13039\/501100004735","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advanced Science"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Photodynamic therapy (PDT) is a promising strategy for the treatment of skin\u2010related tumors including cutaneous squamous cells carcinoma (cSCC). However, it is hard to balance the dosage off\u2010target phototoxicity while maintaining satisfactory therapeutic effect. In addition, oxygen\u2010dependent photosensitizers (PSs) are affected by tumor hypoxic environment, which further causes inefficient photocatalysis and reduces therapeutic effect. Herein, an intelligent DNA nanomachine based on tetrahedral DNA framework is proposed, incorporated with tumor\u2010targeted aptamer and specific miRNA\u2010responsive\u00a0hairpin DNA catalytic assembly (HCA), for precise and high\u2010efficient therapy of cSCC. After aptamer\u2010mediated targeted delivery, a cSCC\u2010specific miRNA selected by tissue sequencing analysis is used to activateHCA, for amplifying PSs and controllably releasing chemotherapeutic drugs. Sequential recognition can greatly improve tumor\u2010specific accumulation and high\u2010dose activation. Moreover, hemin is incorporated into DNA to catalytically produce oxygen. In vitro and in vivo experiments demonstrated that this DNA nanomachine greatly improved anti\u2010tumor effect and realized effective ablation of cSCC in mice, with barely systemic toxicity and inflammation. It is anticipated that this strategy will promote biomedical applications of tumor\u2010specific miRNA and provide a promising option for the non\u2010invasive treatment of skin\u2010associated tumors.<\/jats:p>","DOI":"10.1002\/advs.202415296","type":"journal-article","created":{"date-parts":[[2025,3,23]],"date-time":"2025-03-23T07:15:14Z","timestamp":1742714114000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Targeted DNA Nanomachine Enables Specific miRNA\u2010Responsive Singlet Oxygen Amplification for Precise Cutaneous Squamous Cancer Therapy"],"prefix":"10.1002","volume":"12","author":[{"given":"Hanane","family":"Aliouat","sequence":"first","affiliation":[{"name":"Xiangya School of Pharmaceutical Sciences Central South University  Changsha Hunan 410013 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Detian","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Dermatology Hunan Engineering Research Center of Skin Health and Disease Hunan Key Laboratory of Skin Cancer and Psoriasis Xiangya Hospital Central South University  Changsha Hunan 410008 China"},{"name":"Hangzhou Innovation Institute Beihang University  Hangzhou Zhejiang 310053 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lanyuan","family":"Peng","sequence":"additional","affiliation":[{"name":"Department of Dermatology Hunan Engineering Research Center of Skin Health and Disease Hunan Key Laboratory of Skin Cancer and Psoriasis Xiangya Hospital Central South University  Changsha Hunan 410008 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaxin","family":"Huang","sequence":"additional","affiliation":[{"name":"Xiangya School of Pharmaceutical Sciences Central South University  Changsha Hunan 410013 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongshi","family":"Cheng","sequence":"additional","affiliation":[{"name":"Xiangya School of Pharmaceutical Sciences Central South University  Changsha Hunan 410013 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaojiao","family":"Zhu","sequence":"additional","affiliation":[{"name":"Xiangya School of Pharmaceutical Sciences Central South University  Changsha Hunan 410013 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiang","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Dermatology Hunan Engineering Research Center of Skin Health and Disease Hunan Key Laboratory of Skin Cancer and Psoriasis Xiangya Hospital Central South University  Changsha Hunan 410008 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8846-0532","authenticated-orcid":false,"given":"Nuli","family":"Xie","sequence":"additional","affiliation":[{"name":"Xiangya School of Pharmaceutical Sciences Central South University  Changsha Hunan 410013 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenhu","family":"Zhou","sequence":"additional","affiliation":[{"name":"Xiangya School of Pharmaceutical Sciences Central South University  Changsha Hunan 410013 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuang","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Dermatology Hunan Engineering Research Center of Skin Health and Disease Hunan Key Laboratory of Skin Cancer and Psoriasis Xiangya Hospital Central South University  Changsha Hunan 410008 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,3,21]]},"reference":[{"key":"e_1_2_7_1_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41568-018-0024-5"},{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12032-024-02385-7"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41568-023-00583-5"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers15205037"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201805138"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202217055"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/med.21935"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1039\/D0CS01370F"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.34133\/research.0295"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202402881"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.8b10529"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/EXP.20210265"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.202310199"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.202001718"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.4c03651"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202301814"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/smll.201602770"},{"key":"e_1_2_7_18_1","author":"Li S.","year":"2024","journal-title":"Nat. Protoc"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1039\/C7AN01154G"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.7b02709"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201403236"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.abn2941"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201909345"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s41061-020-0283-y"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202000557"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2022.121532"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.202109609"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0510565103"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.critrevonc.2015.10.003"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202217702"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202305227"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.1c06361"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41596-020-0355-z"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1021\/acssensors.6b00593"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41568-020-00322-0"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2019.11.118"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202109258"}],"container-title":["Advanced Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/advs.202415296","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,6]],"date-time":"2025-10-06T12:16:34Z","timestamp":1759752994000},"score":1,"resource":{"primary":{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/10.1002\/advs.202415296"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3,21]]},"references-count":37,"journal-issue":{"issue":"19","published-print":{"date-parts":[[2025,5]]}},"alternative-id":["10.1002\/advs.202415296"],"URL":"https:\/\/doi.org\/10.1002\/advs.202415296","archive":["Portico"],"relation":{},"ISSN":["2198-3844","2198-3844"],"issn-type":[{"value":"2198-3844","type":"print"},{"value":"2198-3844","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,3,21]]},"assertion":[{"value":"2024-11-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-03-21","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"2415296"}}