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However, maintaining the survival and function of injected or implanted therapeutic cells remains a substantial challenge to success. In vivo modulatory strategy for cell therapeutics has been recently developed, but suffers from limited regenerative efficacy in injured tissue microenvironment with chronic inflammation. Here, an off\u2010the\u2010shelf artificial macrophage (artM) assembled by M2 macrophages\u2010derived lysate proteins\u2010loaded poly (lactic\u2010co\u2010glycolic acid) (PLGA) microspheres coated by macrophage cell membrane is developed. The synthetic artM fabricated in batches maintains its bioactivity with long\u2010term cryostorage. Significantly, artM recapitulates the essential inflammation\u2010regulatory and proregenerative characteristics of endogenous macrophages, including initiating M2 macrophage polarization, resolving excessive inflammation by releasing anti\u2010inflammatory cytokines and growth factors, neutralizing endotoxins and proinflammatory cytokines, augmenting T\u2010helper 2 (T<jats:sub>H<\/jats:sub>2) immune response, and coordinating cell migration and proliferation. In mouse model of deep tissue pressure injury (DTPI), the artM induces tissue regeneration by modulating the inflammatory microenvironment, promoting angiogenesis, reducing scar deposition, and accelerating the renewal of skin appendages. Depletion of macrophages in mice with skin ulcers highlights the immunomodulatory and proangiogenic functions of artM as effective as autogenous macrophages. Collectively, the engineered artM represents a cell\u2010free, proreparative alternative to immune cell therapy in chronic wound management.<\/jats:p>","DOI":"10.1002\/advs.202415886","type":"journal-article","created":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T06:26:22Z","timestamp":1745475982000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["An Off\u2010the\u2010Shelf Artificial Proregenerative Macrophage for Pressure Ulcer Treatment"],"prefix":"10.1002","volume":"12","author":[{"given":"Qi","family":"Su","sequence":"first","affiliation":[{"name":"State Key Laboratory of Advanced Medical Materials and Devices Institute of Biomedical Engineering Chinese Academy of Medical Sciences and Peking Union Medical College  Tianjin 300192 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingrong","family":"Wang","sequence":"additional","affiliation":[{"name":"Beijing Life Science Academy  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