{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T02:57:26Z","timestamp":1792205846020,"version":"4.4.0"},"reference-count":39,"publisher":"Wiley","issue":"17","license":[{"start":{"date-parts":[[2025,3,6]],"date-time":"2025-03-06T00:00:00Z","timestamp":1741219200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,3,6]],"date-time":"2025-03-06T00:00:00Z","timestamp":1741219200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advanced Science"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Less\u2010aggressive lower\u2010grade gliomas (LGGs) frequently transform into glioblastoma (GBM). Most previous studies of gliomas have not focused on LGG\u2010original high\u2010risk subpopulations, which may be one of the most critical hallmarks of glioma progression. In this study, LGG samples are collected to perform single\u2010cell sequencing (scRNA\u2010seq) and identify a unique cell subpopulation marked by CDC20, KIF20A and PTTG1, correlating with poor survival in multiple cohorts. Importantly, the CDC20\n                    <jats:sup>+<\/jats:sup>\n                    KIF20A\n                    <jats:sup>+<\/jats:sup>\n                    PTTG1\n                    <jats:sup>+<\/jats:sup>\n                    cell subpopulation is strongly associated with transforming LGG to GBM according to scRNA\u2010seq and multiplexed immunofluorescence staining assays. In vitro, ex vivo and in vivo investigations further hint that this cell subpopulation is critical to the proliferation and growth of gliomas, and is associated with the hypoxia core activation. Pharmaceutically and therapeutically, the inhibition of this cell subpopulation showed significant anti\u2010tumor effects and effective enhancement of the Temozolomide treatment efficiency. These findings provide insights into the therapeutic strategies of glioma progression, highlighting promising ways to avoid early\u2010stage gliomas developing into advanced gliomas.\n                  <\/jats:p>","DOI":"10.1002\/advs.202416231","type":"journal-article","created":{"date-parts":[[2025,3,11]],"date-time":"2025-03-11T17:19:38Z","timestamp":1741713578000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["The Hypoxia\u2010Associated High\u2010Risk Cell Subpopulation Distinctly Enhances the Progression of Glioma"],"prefix":"10.1002","volume":"12","author":[{"given":"Quan","family":"Wan","sequence":"first","affiliation":[{"name":"Department of Neurosurgery and Emergency Medicine Jiangnan University Medical Center (Wuxi No.2 People's Hospital)  Wuxi Jiangsu 214002 China"},{"name":"Neuroscience Center Wuxi School of Medicine Jiangnan University  Wuxi Jiangsu 214122 China"},{"name":"Wuxi Neurosurgical 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