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Here, a probiotic\u2010based drug delivery system (EcN\n                    <jats:sub>T<\/jats:sub>\n                    @L) is devised to mitigate nanoplastics\u2010induced gut barrier dysfunction by modulating gut immunity and microbiota.\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    Nissle 1917 (EcN) is genetically engineered to produce transforming growth factor\u2010\u03b2 (TGF\u2010\u03b2) and then modified with an Eudragit L100\u201055 coating. This engineered probiotic acts as an in vivo \u201cdrug factory\u201d, exerting anti\u2010inflammatory and immune\u2010regulatory effects, with improved retention and bioavailability in the gastrointestinal tract. EcN\n                    <jats:sub>T<\/jats:sub>\n                    @L effectively protects Caco\u20102 cells from inflammation and infections induced by nano PET, primarily by activating the NF\u2010\u03baB signaling pathway. Besides, EcN\n                    <jats:sub>T<\/jats:sub>\n                    @L demonstrates superior in vivo therapeutic efficacy in treating gastrointestinal infection caused by the combined presence of nano PET and\n                    <jats:italic>Salmonella<\/jats:italic>\n                    , outperforming commercial antibiotics due to its ability to modulate immune responses and gut microbiota. This study highlights the potential of probiotic\u2010based drug delivery systems in addressing nanoplastics\u2010induced gut dysfunctions, offering a promising strategy for mitigating the environmental impact of micro\u2010 and nanoplastics.\n                  <\/jats:p>","DOI":"10.1002\/advs.202417283","type":"journal-article","created":{"date-parts":[[2025,4,2]],"date-time":"2025-04-02T11:00:31Z","timestamp":1743591631000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Engineered Probiotics Mitigate Gut Barrier Dysfunction Induced by Nanoplastics"],"prefix":"10.1002","volume":"12","author":[{"given":"Wenxin","family":"Chen","sequence":"first","affiliation":[{"name":"School of Life and Health Sciences Hainan Province Key Laboratory of One Health Collaborative Innovation Center of One Health Hainan University  Haikou 570228 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiyan","family":"Guo","sequence":"additional","affiliation":[{"name":"School of Life and Health Sciences Hainan Province Key Laboratory of One Health Collaborative Innovation Center of One Health Hainan University  Haikou 570228 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong","family":"Li","sequence":"additional","affiliation":[{"name":"School of Life and Health Sciences Hainan Province Key Laboratory of One Health Collaborative Innovation Center of One Health Hainan University  Haikou 570228 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xue","family":"Chi","sequence":"additional","affiliation":[{"name":"School of Life and Health Sciences Hainan Province Key Laboratory of One Health Collaborative Innovation Center of One Health Hainan University  Haikou 570228 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiang","family":"Ma","sequence":"additional","affiliation":[{"name":"School of Life and Health Sciences Hainan Province Key Laboratory of One Health Collaborative Innovation Center of One Health Hainan University  Haikou 570228 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanqiong","family":"Tang","sequence":"additional","affiliation":[{"name":"School of Life and Health Sciences Hainan Province Key Laboratory of One Health Collaborative Innovation Center of One Health Hainan University  Haikou 570228 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Quanfeng","family":"Liang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Microbial Technology Shandong University  Jinan 250100 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhu","family":"Liu","sequence":"additional","affiliation":[{"name":"Faculty of Animal Science and Technology Key Laboratory of Animal Nutrition and Feed Science of Yunnan Province Yunnan Agricultural University  Kunming 650201 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7616-4931","authenticated-orcid":false,"given":"Yong","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Chemistry and Chemical Engineering Hainan University  Haikou 570228 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0074-1453","authenticated-orcid":false,"given":"Juanjuan","family":"Li","sequence":"additional","affiliation":[{"name":"School of Life and Health Sciences Hainan Province Key Laboratory of One Health Collaborative Innovation Center of One Health Hainan University  Haikou 570228 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,4]]},"reference":[{"key":"e_1_2_9_1_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrgastro.2016.169"},{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2023-331579"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhazmat.2024.134157"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.est.4c01549"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhazmat.2024.133976"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhazmat.2024.135922"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202401009"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12274-024-6757-4"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cej.2024.155527"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41590-018-0230-z"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cgh.2020.11.040"},{"key":"e_1_2_9_12_1","first-page":"188","volume":"12","year":"2015","journal-title":"Nat. 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