{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T23:48:51Z","timestamp":1791935331702,"version":"4.3.4"},"reference-count":34,"publisher":"Wiley","issue":"23","license":[{"start":{"date-parts":[[2025,4,3]],"date-time":"2025-04-03T00:00:00Z","timestamp":1743638400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004739","name":"Youth Innovation Promotion Association of the Chinese Academy of Sciences","doi-asserted-by":"publisher","award":["2023335"],"award-info":[{"award-number":["2023335"]}],"id":[{"id":"10.13039\/501100004739","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advanced Science"],"published-print":{"date-parts":[[2025,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Circulating tumor cells (CTCs) hold significant potential as biomarkers for the diagnosis and management of non\u2010small cell lung cancer (NSCLC). However, their clinical utility is limited by the heterogeneity of CTC subtypes and the need for robust, quantitative assays. In this study, a quantitative CTC RNA assay incorporating multi\u2010antibody\u2010based CTC isolation and specific mRNA quantification by RT\u2010ddPCR is developed. Two distinct models are established: NSCLC CTC Score\n                    <jats:sup>D<\/jats:sup>\n                    for detecting early NSCLC (stages I\u2010II), and NSCLC CTC Score\n                    <jats:sup>M<\/jats:sup>\n                    for monitoring advanced NSCLC (stages III\u2010IV), based on distinct cohort criteria. NSCLC CTC Score\n                    <jats:sup>D<\/jats:sup>\n                    demonstrates high diagnostic performance for early\u2010stage NSCLC, achieving an area under the receiver operating characteristic curve (AUC) of 0.93, significantly outperforming serum CEA (AUC = 0.70). Compared to NSCLC CTC Score\n                    <jats:sup>D<\/jats:sup>\n                    , NSCLC CTC Score\n                    <jats:sup>M<\/jats:sup>\n                    captures a key gene feature of\n                    <jats:italic>KRT19<\/jats:italic>\n                    , whose fragment protein, serum CYFRA 21\u20101, is used as a prognostic biomarker for advanced NSCLC. Notably, CTC Scores\n                    <jats:sup>M<\/jats:sup>\n                    exhibits a more accurate early warning of patient responses to different therapies than serum CYFRA21\u20101 levels, which may provide a potential blood test\u2010based biomarker for improved treatment assessment in advanced NSCLC.\n                  <\/jats:p>","DOI":"10.1002\/advs.202417849","type":"journal-article","created":{"date-parts":[[2025,4,4]],"date-time":"2025-04-04T21:53:18Z","timestamp":1743803598000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Distinct CTC Specific RNA Profile Enables NSCLC Early Detection and Dynamic Monitoring of Advanced NSCLC"],"prefix":"10.1002","volume":"12","author":[{"given":"Xiaoyu","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Pulmonary and Critical Care Medicine The Second Affiliated Hospital of Soochow University  Suzhou 215004 China"},{"name":"CAS Key Laboratory for Nano\u2010Bio Interface Suzhou Institute of Nano\u2010Tech and Nano\u2010Bionics Chinese Academy of Sciences  Suzhou 215123 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pi","family":"Ding","sequence":"additional","affiliation":[{"name":"CAS Key Laboratory for Nano\u2010Bio Interface Suzhou Institute of Nano\u2010Tech and Nano\u2010Bionics Chinese Academy of Sciences  Suzhou 215123 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenjuan","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Pulmonary and Critical Care Medicine The Second Affiliated Hospital of Soochow University  Suzhou 215004 China"},{"name":"CAS Key Laboratory for Nano\u2010Bio Interface Suzhou Institute of Nano\u2010Tech and Nano\u2010Bionics Chinese Academy of Sciences  Suzhou 215123 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Qiu","sequence":"additional","affiliation":[{"name":"CAS Key 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Nano\u2010Tech and Nano\u2010Bionics University of Science and Technology of China  Hefei 230026 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhili","family":"Wang","sequence":"additional","affiliation":[{"name":"CAS Key Laboratory for Nano\u2010Bio Interface Suzhou Institute of Nano\u2010Tech and Nano\u2010Bionics Chinese Academy of Sciences  Suzhou 215123 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheng","family":"Li","sequence":"additional","affiliation":[{"name":"CAS Key Laboratory for Nano\u2010Bio Interface Suzhou Institute of Nano\u2010Tech and Nano\u2010Bionics Chinese Academy of Sciences  Suzhou 215123 China"},{"name":"School of Nano\u2010Tech and Nano\u2010Bionics University of Science and Technology of China  Hefei 230026 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yufei","family":"Xing","sequence":"additional","affiliation":[{"name":"Department of Pulmonary and Critical Care Medicine The Second Affiliated Hospital of Soochow University  Suzhou 215004 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingjing","family":"Shen","sequence":"additional","affiliation":[{"name":"Thoracic Department The Second Affiliated Hospital of Soochow University  Suzhou 215004 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yawen","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Pulmonary and Critical Care Medicine The Second Affiliated Hospital of Soochow University  Suzhou 215004 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Pulmonary and Critical Care Medicine The Second Affiliated Hospital of Soochow University  Suzhou 215004 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