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Herein, a theranostic nanoplatform for the second near\u2010infrared window (NIR\u2010II) fluorescence imaging\u2010guided membrane\u2010targeted mild photothermal\u2010immunotherapy of glioblastoma using genetically engineered CSF1R\/IL12\u2010macrophage membrane (MM)\u2010liposome hybrid nanovesicles, is reported. By mimicking lipophilic membrane probe (Dil) with octadecyl chains, a NIR\u2010II emissive photothermal dye (\n                    <jats:bold>IRC18<\/jats:bold>\n                    ), which realizes labeling of nanovesicle lipid bilayers for biodistribution tracing, glioblastoma diagnosis, and molecular imaging of tumoral microenvironment, is synthesized. Importantly, MM and c\u2010RGD\u2010decorated liposome together offer BBB crossing, tumor targeting, and long\u2010term circulation; while, the genetically overexpressed CSF1R and IL12 on MM surface contribute to effective modulation of M2\u2010to\u2010M1 macrophage repolarization and local promotion of T cell cytotoxicity in glioblastoma microenvironment, respectively. Notably, through membrane fusion,\n                    <jats:bold>IRC18<\/jats:bold>\n                    dyes translocate from nanovesicle lipid bilayers to glioblastoma membranes, which achieve membrane\u2010targeted mild photothermal therapy to ablate primary tumor and induce immunogenic cell death to promote antigen presentation. More importantly, the combined blockade of the CSF1\u2010CSF1R axis and IL\u201012 enrichment not only reprograms the tumor microenvironment through macrophage M1 repolarization but also activates cytotoxic T cells, ultimately achieving complete glioblastoma eradication. This research provides an efficient theranostic paradigm for glioblastoma treatment.\n                  <\/jats:p>","DOI":"10.1002\/advs.202500131","type":"journal-article","created":{"date-parts":[[2025,4,25]],"date-time":"2025-04-25T16:11:13Z","timestamp":1745597473000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Genetically Engineered IL12\/CSF1R\u2010Macrophage Membrane\u2010Liposome Hybrid Nanovesicles for NIR\u2010II Fluorescence Imaging\u2010Guided and Membrane\u2010Targeted Mild Photothermal\u2010Immunotherapy of Glioblastoma"],"prefix":"10.1002","volume":"12","author":[{"given":"Pengfei","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Traumatic Orthopedics Shenzhen Longhua District Central Hospital  Shenzhen 518110 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Science 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