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This highlights the need for further optimization of culture conditions. Through compound library screening, we identified Danofloxacin, a small molecule, as a promoter of mouse ESC stemness. Danofloxacin enhances the formation of undifferentiated colonies and the generation of chimeric mice. Mechanistically, it suppresses\n                    <jats:italic>HDAC1<\/jats:italic>\n                    expression and activity, resulting in increased acetylation of histone H3 at lysines 9 (H3K9ac) and 27 (H3K27ac), which in turn upregulates the expression of\n                    <jats:italic>Tert<\/jats:italic>\n                    and\n                    <jats:italic>Prdm10<\/jats:italic>\n                    . Functional assays revealed that overexpressing\n                    <jats:italic>Tert<\/jats:italic>\n                    or\n                    <jats:italic>Prdm10<\/jats:italic>\n                    mimics Danofloxacin's effect on ESC self\u2010renewal, whereas knockdown of either gene disrupts this process. Moreover, the elevated levels of H3K9ac and H3K27ac activate the PI3K\/AKT and HIF\u20101\u03b1 signaling pathways, both of which contribute to the transcriptional upregulation of\n                    <jats:italic>Tert<\/jats:italic>\n                    and\n                    <jats:italic>Prdm10<\/jats:italic>\n                    . These findings offer new insights into the pluripotency regulatory network and propose potential strategies for isolating ESCs from other species in vitro.\n                  <\/jats:p>","DOI":"10.1002\/advs.202500624","type":"journal-article","created":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T18:00:31Z","timestamp":1790532031000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Chemical Screening Identifies Danofloxacin as a Self\u2010Renewal Agonist of Embryonic Stem Cells through Alleviation of HDAC1\u2010Mediated Deacetylation of\n                    <i>Tert<\/i>\n                    and\n                    <i>Prdm10<\/i>"],"prefix":"10.1002","author":[{"given":"Yan","family":"Zhang","sequence":"first","affiliation":[{"name":"Center for Stem Cell and Translational Medicine School of Life Sciences and Medical Engineering Anhui University  Hefei China"},{"name":"Shanghai Key Laboratory of Maternal 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