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Nonetheless, the precise mechanisms underlying alterations in the tumor microenvironment (TME) in LN metastasis in breast cancer remain poorly understood. Single\u2010cell RNA sequencing of 28 LN samples from 23 patients is performed, and a comprehensive landscape of the entire ecosystem is generated. Ten major cell types are identified, with the subclusters of each major cell type exhibiting diverse characteristics. Furthermore, multiple signatures are collected to evaluate the key components of the subclusters using multi\u2010omics methodologies. This study finds that myCAFs may hasten LN metastasis, and observed a notable increase in\n                    <jats:italic>APOE<\/jats:italic>\n                    + macrophages and a higher proportion of exhausted\n                    <jats:italic>CD8<\/jats:italic>\n                    + T cells, contributing to the immunosuppressive TME. Moreover, cancer cells in metastatic lesions exhibited diverse expression patterns linked to proliferation, metastasis, oxidative phosphorylation, hypoxia, and interferon responses. Using multi\u2010omics approaches and experimental validations, it determines that\n                    <jats:italic>GLO1<\/jats:italic>\n                    can promote lymphatic angiogenesis, metastasis, and inhibit the proteasomal degradation of\n                    <jats:italic>GSS<\/jats:italic>\n                    , thereby maintaining intracellular glutathione (GSH) and reactive oxygen species (ROS) balance. Collectively, the study offers novel perspectives on the microenvironment remodeling of breast cancer LN metastases, suggesting that\n                    <jats:italic>GLO1<\/jats:italic>\n                    may be a promising therapeutic target.\n                  <\/jats:p>","DOI":"10.1002\/advs.202500722","type":"journal-article","created":{"date-parts":[[2025,6,10]],"date-time":"2025-06-10T05:27:30Z","timestamp":1749533250000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["Paracrine Orchestration of Tumor Microenvironment Remodeling Induced by GLO1 Potentiates Lymph Node Metastasis in Breast Cancer"],"prefix":"10.1002","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2482-7358","authenticated-orcid":false,"given":"Jindong","family":"Xie","sequence":"first","affiliation":[{"name":"State Key Laboratory of Oncology in South China Guangdong Provincial Clinical Research Center for Cancer Sun 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