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Curtailing these functions is essential in therapies such as chimeric antigen receptor T\u2010cell (CAR\u2010T) therapies or organ transplantation to avoid hyperactivation and rejection. A major challenge in the field is the precise, live measurement of T cell function at the single\u2010cell level, limiting the prediction of immune responses, the development of effective immunotherapies, and optimization of immunosuppressive regimens. Gamma\u2010Glutamyl Cysteine Ligase (GCL), the rate\u2010limiting enzyme in glutathione (GSH) synthesis, is essential for T cell function in mice, but its role in human T cells is underexplored.\n                    <jats:bold>GLed<\/jats:bold>\n                    , a novel reversible lanthanide\u2010based GSH sensor is introduced that enables real\u2010time, quantitative measurements of GCL activity at single\u2010cell resolution. The\n                    <jats:bold>GLed<\/jats:bold>\n                    approach distinguishes GSH contributions from GCL and GSR, linking GCL activity directly to human T cell effector functions. Additionally, this reveals previously unknown modulation of GCL activity by immunosuppressive drugs, underscoring GCL as a critical player in T cell function and a potential therapeutic target in immune\u2010related diseases.\n                  <\/jats:p>","DOI":"10.1002\/advs.202501179","type":"journal-article","created":{"date-parts":[[2025,7,13]],"date-time":"2025-07-13T12:17:58Z","timestamp":1752409078000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Gamma\u2010Glutamyl Cysteine Ligase Activity as a Proxy for Human T Cell Function and Drug\u2010Induced Immunosuppression"],"prefix":"10.1002","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6837-3928","authenticated-orcid":false,"given":"Francisco","family":"Fueyo\u2010Gonz\u00e1lez","sequence":"first","affiliation":[{"name":"Instituto de Qu\u00edmica M\u00e9dica (IQM\u2010CSIC)  Juan de la Cierva 3 Madrid 28006 Spain"},{"name":"Department of Pharmacological Sciences Center of Translational Medicine and Pharmacology Icahn School of Medicine at Mount Sinai  New York NY 10029 USA"},{"name":"Department of Medicine Translational Transplant Research Center Immunology Institute Icahn School of Medicine at Mount Sinai  New York NY 10029 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carmen","family":"Salto\u2010Giron","sequence":"additional","affiliation":[{"name":"Nanoscopy\u2010UGR Laboratory. 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Departamento de Fisicoqu\u00edmica Unidad de Excelencia de Qu\u00edmica Aplicada a Biomedicina y Medioambiente Facultad de Farmacia Universidad de Granada Campus Cartuja  Granada 18071 Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0273-2761","authenticated-orcid":false,"given":"Rosario","family":"Herranz","sequence":"additional","affiliation":[{"name":"Instituto de Qu\u00edmica M\u00e9dica (IQM\u2010CSIC)  Juan de la Cierva 3 Madrid 28006 Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1905-4183","authenticated-orcid":false,"given":"Angel","family":"Orte","sequence":"additional","affiliation":[{"name":"Nanoscopy\u2010UGR Laboratory. 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