{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T13:00:48Z","timestamp":1790254848197,"version":"4.1.0"},"reference-count":55,"publisher":"Wiley","issue":"43","license":[{"start":{"date-parts":[[2025,8,30]],"date-time":"2025-08-30T00:00:00Z","timestamp":1756512000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,8,30]],"date-time":"2025-08-30T00:00:00Z","timestamp":1756512000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["No. 82401720"],"award-info":[{"award-number":["No. 82401720"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advanced Science"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Central retinal artery occlusion (CRAO) can lead to retinal ischemia (RI), resulting in painless vision impairment. M\u00fcller cells, the principal supporting glial cells of the retina, are distributed throughout its layers and play pivotal roles in maintaining retinal homeostasis by regulating inflammation, oxidative stress, and angiogenesis following RI. However, the specific role of M\u00fcller cells in RI induced by middle cerebral artery occlusion (MCAO) remains unclear. In this study, single\u2010nucleus RNA sequencing is employed to investigate transcriptional changes in various retinal cell types following MCAO\u2010induced RI. A novel M\u00fcller cell subpopulation, characterized by the highest expression of the high\u2010mobility group A2 gene (\n                    <jats:italic>Hmga2<\/jats:italic>\n                    ), emerged after RI. Knockout of\n                    <jats:italic>Hmga2<\/jats:italic>\n                    alleviated neuroinflammation and RI\u2010related symptoms, potentially through binding to phosphoinositide 3\u2010kinase and regulating M\u00fcller cell autophagy. Based on these findings, a targeted strategy using hybrid nanoparticles composed of M\u00fcller cell membranes and liposomes\u2014termed siRNA\u2010Hmga2@LMM\u2014is developed to deliver siRNA against Hmga2. In vivo experiments revealed that intravitreal injection of siRNA\u2010Hmga2@LMM improved retinal function following RI. These findings provide new mechanistic insights and identify potential targets for the treatment of RI.\n                  <\/jats:p>","DOI":"10.1002\/advs.202502534","type":"journal-article","created":{"date-parts":[[2025,8,30]],"date-time":"2025-08-30T10:00:32Z","timestamp":1756548032000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Single\u2010Cell Transcriptome Analysis Reveals That Hmga2 Regulates Neuroinflammation and Retinal Function by Modulating M\u00fcller Cell Autophagy Through PI3K\/AKT Signaling Following MCAO\u2010Induced Retinal Ischemia"],"prefix":"10.1002","volume":"12","author":[{"given":"Weihao","family":"Lv","sequence":"first","affiliation":[{"name":"Department of Neurosurgery Xijing Hospital Fourth Military Medical University  Xi'an Shaanxi 710032 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juzheng","family":"Yuan","sequence":"additional","affiliation":[{"name":"Department of General Surgery Xijing Hospital Fourth Military Medical University  Xi'an Shaanxi Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhe","family":"Ruan","sequence":"additional","affiliation":[{"name":"Department of Neurology Tangdu Hospital Fourth Military Medical University  Xi'an Shaanxi 710032 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ya\u2010nan","family":"Dou","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery Xijing Hospital Fourth Military Medical University  Xi'an Shaanxi 710032 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongchen","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery Xijing Hospital Fourth Military Medical University  Xi'an Shaanxi 710032 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaowei","family":"Fei","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery Xijing Hospital Fourth Military Medical University  Xi'an Shaanxi 710032 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leiying","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology Xijing Hospital Fourth Military Medical University  Xi'an Shaanxi 710032 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zehan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery Xijing Hospital Fourth Military Medical University  Xi'an Shaanxi 710032 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of General Surgery Tangdu Hospital Fourth Military Medical University  Xi'an Shaanxi 710032 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiuquan","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery Xijing Hospital Fourth Military Medical University  Xi'an Shaanxi 710032 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhou","family":"Fei","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery Xijing Hospital Fourth Military Medical University  Xi'an Shaanxi 710032 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6194-5663","authenticated-orcid":false,"given":"Fei","family":"Fei","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology Xijing Hospital Fourth Military Medical University  Xi'an Shaanxi 710032 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,8,30]]},"reference":[{"key":"e_1_2_8_1_1","volume":"52","author":"Grory B. M.","year":"2021","journal-title":"Stroke"},{"key":"e_1_2_8_1_2","first-page":"Cd00","volume":"1","author":"Lin J. C.","year":"2023","journal-title":"The Cochrane database of systematic reviews"},{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9394(99)00359-1"},{"key":"e_1_2_8_2_2","doi-asserted-by":"publisher","DOI":"10.1161\/STROKEAHA.123.045957"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00415-022-11439-7"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.4103\/1673-5374.270293"},{"key":"e_1_2_8_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/14651858.CD008390.pub2"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1161\/STROKEAHA.107.504357"},{"key":"e_1_2_8_5_2","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2017.00254"},{"key":"e_1_2_8_5_3","volume":"24","author":"Lv W.","year":"2023","journal-title":"Int. J. Mol. Sci."},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.preteyeres.2015.06.003"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2022.1047487"},{"key":"e_1_2_8_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.preteyeres.2006.05.003"},{"key":"e_1_2_8_7_3","doi-asserted-by":"publisher","DOI":"10.1002\/glia.22380"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-23601-1"},{"key":"e_1_2_8_8_2","first-page":"1","volume":"30","author":"Feng Z.","year":"2024","journal-title":"Molecular vision"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxlet.2017.10.018"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12886-023-03022-z"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0068773"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12974-021-02366-x"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1080\/14728222.2022.2072730"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-09234-6"},{"key":"e_1_2_8_14_2","first-page":"2537","volume":"117","author":"Vallejo J.","year":"2021","journal-title":"Cardiovasc. Res."},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.addr.2007.03.009"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1124\/pr.120.019554"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.4155\/tde-2019-0009"},{"key":"e_1_2_8_18_1","volume":"11","author":"Lin R. R.","year":"2024","journal-title":"Adv. Sci. (Weinheim, Baden\u2010Wurttemberg, Germany)"},{"key":"e_1_2_8_19_1","first-page":"775","volume":"55","author":"Zhang S.","year":"2019","journal-title":"International journal of oncology"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.65411"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1155\/2022\/1376959"},{"key":"e_1_2_8_22_1","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1186\/s13046-019-1183-2","volume":"38","author":"Yang K.","year":"2019","journal-title":"Journal of experimental & clinical cancer research : CR"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnins.2021.642176"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12974-024-03175-8"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/cns.13084"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.4103\/1673-5374.339500"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvab237"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nbd.2024.106671"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioactmat.2021.01.032"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41571-022-00699-x"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2015.07.019"},{"key":"e_1_2_8_31_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.nanolett.7b05263"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymthe.2024.01.005"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1021\/mp500388m"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.6b06040"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.202304285"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.nanolett.4c05012"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00011-023-01824-x"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-017-1382-0"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-019-0619-0"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1089\/omi.2011.0118"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-14-7"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.4402"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-018-0414-6"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1111\/joa.12653"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-0716-3441-7_5"}],"container-title":["Advanced Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/advs.202502534","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/advs.202502534","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/advs.202502534","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T12:04:23Z","timestamp":1790251463000},"score":1,"resource":{"primary":{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/10.1002\/advs.202502534"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,8,30]]},"references-count":55,"journal-issue":{"issue":"43","published-print":{"date-parts":[[2025,11]]}},"alternative-id":["10.1002\/advs.202502534"],"URL":"https:\/\/doi.org\/10.1002\/advs.202502534","archive":["Portico"],"relation":{},"ISSN":["2198-3844","2198-3844"],"issn-type":[{"value":"2198-3844","type":"print"},{"value":"2198-3844","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,8,30]]},"assertion":[{"value":"2025-02-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-08-30","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e02534"}}