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This study presents an integrated miniature blood processing and mass spectrometry (MS) analysis system, which incorporates automated magnetic solid\u2010phase extraction, self\u2010aspiration sampling miniature mass spectrometer, and deep learning algorithms for automated quantitative analysis. It achieves full automation from sample preparation to detection, demonstrating the capability to analyze serum psychoactive drugs with a 15\u2010second\/MS acquisition and 8\u2010sample parallel processing within 30 minutes (including pretreatment). This has significantly increased detection throughput and facilitated the establishment of the standard curve. The novel dual\u2010target ion parallel tandem MS analysis technique, combined with a U\u2010net peak area recognition algorithm, achieved over 98% identification accuracy with less than 0.2% area prediction deviation. Quantitative analysis showed high correlation coefficients &gt;0.99 in medically relevant ranges, supported by relative standard deviation &lt; 10% and average back\u2010calculated accuracy deviation &lt; 3.5%. Clinical validation revealed strong concordance with LC\u2010MS\/MS. The system's integration of automated sample processing, miniature MS hardware, and AI\u2010driven data analysis establishes a paradigm for high\u2010throughput clinical detection. The advantages of accuracy, automation, intelligence, miniaturization, and high throughput suggest significant potential for this system in clinical detection and personalized medicine.<\/jats:p>","DOI":"10.1002\/advs.202502721","type":"journal-article","created":{"date-parts":[[2025,5,23]],"date-time":"2025-05-23T03:55:33Z","timestamp":1747972533000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Fully Automated Deep Learning Enabled Miniature Mass Spectrometry System for Psychoactive Therapeutic Drug Monitoring"],"prefix":"10.1002","volume":"12","author":[{"given":"Yuanhao","family":"Zhou","sequence":"first","affiliation":[{"name":"School of Biomedical Engineering Shenzhen Campus of Sun Yat\u2010sen University  Shenzhen 518107 China"},{"name":"Key Laboratory of Sensing Technology and Biomedical Instruments of Guangdong Province School of Biomedical Engineering Sun Yat\u2010sen University  Guangzhou 510275 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiawen","family":"Ai","sequence":"additional","affiliation":[{"name":"Division of Advanced Manufacturing Graduate School at Shenzhen Tsinghua University  Shenzhen 518055 China"},{"name":"State Key Laboratory of Precision Measurement Technology and Instruments Department of Precision Instrument Tsinghua University  Beijing 100084 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zi","family":"Ye","sequence":"additional","affiliation":[{"name":"School of Biomedical Engineering Shenzhen Campus of Sun Yat\u2010sen University  Shenzhen 518107 China"},{"name":"Key Laboratory of Sensing Technology and Biomedical Instruments of Guangdong Province School of Biomedical Engineering Sun Yat\u2010sen University  Guangzhou 510275 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kexin","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Biomedical Engineering Shenzhen Campus of Sun Yat\u2010sen University  Shenzhen 518107 China"},{"name":"Key Laboratory of Sensing Technology and Biomedical Instruments of Guangdong Province School of Biomedical Engineering Sun Yat\u2010sen University  Guangzhou 510275 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"JingXiong","family":"Lin","sequence":"additional","affiliation":[{"name":"School of Biomedical Engineering Shenzhen Campus of Sun Yat\u2010sen University  Shenzhen 518107 China"},{"name":"Key Laboratory of Sensing Technology and Biomedical Instruments of Guangdong Province School of Biomedical Engineering Sun Yat\u2010sen University  Guangzhou 510275 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenhua","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences (Shenzhen) Shenzhen Campus of Sun Yat\u2010sen University  Shenzhen 518107 China"},{"name":"Shenzhen Key Laboratory of Chinese Medicine Active Substance Screening and Translational Research Department of Pharmacy, The Seventh Affiliated Hospital Sun Yat\u2010sen University  Shenzhen 518107 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mi","family":"Luo","sequence":"additional","affiliation":[{"name":"CHIN Instrument (Hefei) Co., Ltd.  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