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However, the hepatic anatomic structures and their pathological alterations in the whole\u2010liver scale remain to be elucidated. Combining the micro\u2010optical sectioning tomography (MOST) system and liver Nissl staining, a first high\u2010precision whole mouse liver atlas is generated, enabling visualization and analysis of the entire mouse liver. Thus, a detailed 3D panorama of CCl4\u2010induced liver fibrosis pathology is constructed, capturing the 3D details of the central veins, portal veins, arteries, bile ducts, hepatic sinusoids, and liver cells. Pathological changes, including damaged sinusoids, steatotic hepatocytes, and collagen deposition, are region\u2010specific and concentrated in the pericentral areas. The quantitative analysis shows a significantly reduced diameter and increased length density of the central vein. Additionally, a deep learning tool is used to segment steatotic hepatocytes, finding that the volume proportion of steatotic regions is similar across liver lobes. Steatosis severity increases with proximity to the central vein, independent of central vein diameter. The approach allows the cross\u2010scale visualization of multiple structural components in liver research and promotes pathological studies from a 2D to a 3D perspective.<\/jats:p>","DOI":"10.1002\/advs.202502744","type":"journal-article","created":{"date-parts":[[2025,3,25]],"date-time":"2025-03-25T17:45:37Z","timestamp":1742924737000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Generation of a High\u2010Precision Whole Liver Panorama and Cross\u2010Scale 3D Pathological Analysis for Hepatic Fibrosis"],"prefix":"10.1002","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9315-5065","authenticated-orcid":false,"given":"Xiaochuan","family":"Zhang","sequence":"first","affiliation":[{"name":"Center for MOST and Image Fusion Analysis Shanghai Institute of Materia Medica Chinese Academy of Sciences  Shanghai 201210 China"},{"name":"CAS Key Laboratory of Drug Research Shanghai Institute of Materia 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