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Activation of hepatic stellate cells (HSCs) is a key process in liver fibrosis. This study presents a live\u2010cell drug\u2010screening approach that specifically targets fibrosis\u2010associated collagen type I alpha 1 (\n                    <jats:italic>COL1A1<\/jats:italic>\n                    ) mRNA in activated HSCs through the use of fluorogenic RNA self\u2010assembly. It employs a dual\u2010probe system to construct an RNA Mango II structure, which upon binding with\n                    <jats:italic>COL1A1<\/jats:italic>\n                    mRNA, facilitates activation of the TO1\u2010Biotin fluorophore, thereby enabling the visualization of mRNA to indicate HSC activation levels. Through the application of a high throughput live\u2010cell screening system, dihydrotanshinone I (DHT) is identified as potent leading antifibrotic compound, evidenced by its inhibitory effects on\n                    <jats:italic>COL1A1<\/jats:italic>\n                    mRNA expression. The therapeutic efficacy of DHT is further substantiated by monitoring\n                    <jats:italic>COL1A1<\/jats:italic>\n                    mRNA dynamics following treatment. In\n                    <jats:italic>vivo<\/jats:italic>\n                    studies demonstrates the sustained administration of DHT significantly ameliorated liver fibrosis in mice models. This method offers a simple, cost\u2010effective approach of visualizing RNA dynamics and conducting drug screening in live cells, presenting a significant potential for the development of hepatic fibrosis therapies.\n                  <\/jats:p>","DOI":"10.1002\/advs.202502850","type":"journal-article","created":{"date-parts":[[2025,5,23]],"date-time":"2025-05-23T08:00:34Z","timestamp":1747987234000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["In Situ Assembly of Fluorogenic RNA for Screening Natural Anti\u2010Liver Fibrosis Products via Dynamic Visualization of\n                    <i>COL1A1<\/i>\n                    mRNA"],"prefix":"10.1002","volume":"12","author":[{"given":"Rui","family":"Bai","sequence":"first","affiliation":[{"name":"School of Materials Science and Engineering Suzhou University of Science and Technology  Kerui Road Suzhou 215009 P. R. 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China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0956-2777","authenticated-orcid":false,"given":"Bin","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biomolecular Engineering National University of Singapore  Singapore 117585 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