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While surgical resection removes the primary tumor, it often fails to prevent recurrence or metastasis, and despite the promise of immunotherapy, response to immune checkpoint blockade remains poor. Here, a cryogel\u2010based dendritic cell (DC) immunotherapy is developed incorporating gold nanodot\u2010lipopolysaccharide (AuLPS)\u2010loaded DCs, doxorubicin (Dox), and PD\u20101 immune checkpoint blockade (aPD\u20101+Dox+AuLPS@DC) to enhance post\u2010surgical antitumor immunity. The AuLPS nanoparticles (NPs) stabilize LPS assembly, optimizing Th1 adjuvant activity and improving DC immunotherapy efficacy while minimizing adverse effects. The cryogel enables the sustained, localized release of therapeutic agents at the surgical site, preserving DC viability, migration, and functionality within the tumor microenvironment. This strategy enhances DC activation and potentiates robust T\u2010cell activation in both tumor\u2010draining lymph nodes and tumor beds, leading to durable antitumor immunity. When administered at the post\u2010surgical site in an orthotopic TNBC model, the aPD\u20101+Dox+AuLPS@DC cryogel immunotherapy significantly delays tumor recurrence, reduces distant metastasis, and prolongs survival. These findings highlight cryogel\u2010based DC immunotherapy as a promising post\u2010surgical therapeutic strategy to enhance responses to immune checkpoint blockade and improve outcomes in TNBC.<\/jats:p>","DOI":"10.1002\/advs.202503238","type":"journal-article","created":{"date-parts":[[2025,7,11]],"date-time":"2025-07-11T10:24:24Z","timestamp":1752229464000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Cryogel\u2010Based Dendritic Cell Immunotherapy for Post\u2010Surgical Breast Cancer Treatment"],"prefix":"10.1002","volume":"12","author":[{"given":"Lam\u2010Duc\u2010Huy","family":"Nguyen","sequence":"first","affiliation":[{"name":"Institute of Biomedical Engineering National Tsing Hua University  Hsinchu 30013 Taiwan"},{"name":"Department of Chemical Engineering National Tsing Hua University  Hsinchu 34881 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