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Despite the identification of molecular alterations in ACP, the absence of robust research models has impeded the advancement of targeted therapies. Herein, the development of a large\u2010scale ACP biobank comprising 54 patient\u2010derived organoids (PDOs) is presented, achieves with a notable 90% success rate. Comprehensive characterization using hematoxylin and eosin (H&amp;E) staining, immunofluorescence staining, and whole\u2010exome sequencing (WES) demonstrates that PDOs faithfully recapitulate key histoarchitectural features, molecular marker expression profiles, and somatic mutational landscapes of corresponding parental tumors. Drug sensitivity screening reveals diverse responses of PDOs to the drugs tested, with Ceritinib exhibiting potent and consistent anti\u2010tumor activity across seventeen PDOs evaluated. Further mechanistic investigations utilizing RNA transcriptomic sequencing have elucidated that Ceritinib inhibits PDO growth by downregulating the IGF\u20101R\/PI3K\/AKT\/GSK\u20103\u03b2\/\u03b2\u2010catenin signaling axis. Additionally, a retrospective analysis of two Ceritinib\u2010treated clinical cases reveals tumor growth with treatment before any possible therapeutic effects are observed, highlighting the need for caution and careful monitoring in treating ACP patients. Collectively, these findings demonstrate that ACP PDOs effectively preserve the biological characteristics of original tumors, thereby providing a valuable platform for developing precision therapies for ACP patients.<\/jats:p>","DOI":"10.1002\/advs.202503924","type":"journal-article","created":{"date-parts":[[2025,11,18]],"date-time":"2025-11-18T09:32:16Z","timestamp":1763458336000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["A Patient\u2010Derived Organoid Biobank of Adamantinomatous Craniopharyngioma as a Platform for Drug Discovery"],"prefix":"10.1002","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1361-4434","authenticated-orcid":false,"given":"Huarong","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Neurosurgery Nanfang Hospital Southern Medical University  Guangzhou Guangdong 510515 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