{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T13:57:45Z","timestamp":1791986265866,"version":"4.4.0"},"reference-count":50,"publisher":"Wiley","issue":"27","license":[{"start":{"date-parts":[[2025,5,5]],"date-time":"2025-05-05T00:00:00Z","timestamp":1746403200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,5,5]],"date-time":"2025-05-05T00:00:00Z","timestamp":1746403200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advanced Science"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Synthetic biology has enabled the development of biosensors to detect intestinal inflammation, yet few target the clinically validated biomarker of intestinal inflammation calprotectin with both diagnostic and therapeutic capabilities. Here, an optimized calprotectin biosensor is presented that leverages a zinc uptake regulator (Zur) controlled promoter coupled with a memory circuit to detect and record intestinal inflammation in vivo. The level of biosensor activation strongly correlates with calprotectin levels in the colon of two independent mouse models of colitis. Coupling of the biosensor with the production of the anti\u2010inflammatory cytokine IL10 allowed for the resolution of chemically induced colitis, demonstrating the ability of the biosensor to sense and respond to disease. This work highlights the utility of developing synthetic organisms for the diagnosis and treatment of intestinal disease using clinically validated biomarkers.<\/jats:p>","DOI":"10.1002\/advs.202504364","type":"journal-article","created":{"date-parts":[[2025,5,5]],"date-time":"2025-05-05T09:56:28Z","timestamp":1746438988000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Microbial Biosensor for Sensing and Treatment of Intestinal Inflammation"],"prefix":"10.1002","volume":"12","author":[{"given":"Duolong","family":"Zhu","sequence":"first","affiliation":[{"name":"Department of Molecular Virology and Microbiology Baylor College of Medicine  Houston 77030 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey","family":"Galley","sequence":"additional","affiliation":[{"name":"Department of Molecular Virology and Microbiology Baylor College of Medicine  Houston 77030 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jason","family":"Pizzini","sequence":"additional","affiliation":[{"name":"Department of Molecular Virology and Microbiology Baylor College of Medicine  Houston 77030 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"Musteata","sequence":"additional","affiliation":[{"name":"Systems, Synthetic, and Physical Biology Ph.D. Program Rice University  Houston 77005 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin V.","family":"Douglas","sequence":"additional","affiliation":[{"name":"Department of Pathology Vanderbilt Institute for Infection Immunology, and Inflammation Microbiology, and Immunology Vanderbilt University  Nashville 37235 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Walter J.","family":"Chazin","sequence":"additional","affiliation":[{"name":"Departments of Biochemistry and Chemistry Vanderbilt University  Nashville 37235 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric P.","family":"Skaar","sequence":"additional","affiliation":[{"name":"Department of Pathology Vanderbilt Institute for Infection Immunology, and Inflammation Microbiology, and Immunology Vanderbilt University  Nashville 37235 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey J.","family":"Tabor","sequence":"additional","affiliation":[{"name":"Department of Bioengineering Department of Biosciences, Systems Synthetic, and Physical Biology Ph.D. Program Rice Synthetic Biology Institute Rice University  Houston 77005 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8983-9539","authenticated-orcid":false,"given":"Robert A.","family":"Britton","sequence":"additional","affiliation":[{"name":"Department of Molecular Virology and Microbiology Alkek Center for Metagenomics and Microbiome Research Dan L. 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