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The organ\u2010on\u2010chip technology has achieved great advances in disease modeling, yet its potential in EMs\u2010related research remains largely untapped. Herein, a microfluidic chip platform that integrates primary cell\u2010laden microcapsules for personalized drug evaluation. Specifically, primary human ectopic endometrial stromal cells (hESCs) within microcapsules featuring a biocompatible carboxymethyl cellulose (CMC) core and a stable alginate (ALG) shell using precise microfluidic electrospray are encapsulated. These microcapsules are integrated into a chip with a branched gradient generator and multiple cell\u2010culture chambers, enabling tailored and high\u2010throughput drug screening. By exposing hESCs\u2010microcapsules derived from primary cells of distinct patient individuals to various drugs on\u2010chip, significant inter\u2010individual variability was revealed, with a strong correlation to clinical outcomes. This unique combination of patient\u2010specific 3D microenvironments and dynamic drug gradient control represents a paradigm shift in personalized EMs research. Further integrating with omics techniques, its capability in exploring promising drugs is showcased. These results reveal that the chip platform could deliver dependable and personalized drug screening outcomes, thereby benefiting both scientific inquiries and clinical therapies.<\/jats:p>","DOI":"10.1002\/advs.202504647","type":"journal-article","created":{"date-parts":[[2025,4,28]],"date-time":"2025-04-28T05:26:37Z","timestamp":1745817997000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Drug Screening of Primary Human Endometriotic Cells Based on Micro\u2010Encapsulating Microfluidic Chip"],"prefix":"10.1002","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9877-460X","authenticated-orcid":false,"given":"Qiong","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University  Wenzhou 325027 China"},{"name":"Oncology Discipline Group The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University  Wenzhou 325027 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7110-4841","authenticated-orcid":false,"given":"Jing","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University  Wenzhou 325027 China"},{"name":"Oncology Discipline Group The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University  Wenzhou 325027 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenzhao","family":"Li","sequence":"additional","affiliation":[{"name":"Shanghai Xuhui Central Hospital Zhongshan\u2010Xuhui Hospital and the Shanghai Key Laboratory of Medical Epigenetics International Co\u2010laboratory of Medical Epigenetics and Metabolism (Ministry of Science and Technology Institutes of Biomedical Sciences) Fudan University  Shanghai 200032 China"},{"name":"Wenzhou Institute University of Chinese Academy of Sciences  Wenzhou 325001 China"},{"name":"Department of Biomedical Engineering The Hong Kong Polytechnic University  Hong Kong SAR 999077 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7458-9100","authenticated-orcid":false,"given":"Luoran","family":"Shang","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University  Wenzhou 325027 China"},{"name":"Oncology Discipline Group The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University  Wenzhou 325027 China"},{"name":"Shanghai Xuhui Central Hospital Zhongshan\u2010Xuhui Hospital and the Shanghai Key Laboratory of Medical Epigenetics International Co\u2010laboratory of Medical Epigenetics and Metabolism (Ministry of Science and Technology Institutes of Biomedical Sciences) Fudan University  Shanghai 200032 China"},{"name":"Wenzhou Institute University of Chinese Academy of Sciences  Wenzhou 325001 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dexuan","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Pediatrics The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University  Wenzhou 325027 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ping","family":"Duan","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University  Wenzhou 325027 China"},{"name":"Oncology Discipline Group The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University  Wenzhou 325027 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,4,28]]},"reference":[{"key":"e_1_2_8_1_1","doi-asserted-by":"crossref","DOI":"10.1136\/bmj-2022-070750","volume":"379","author":"Horne A. 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