{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T19:38:07Z","timestamp":1790969887152,"version":"4.1.0"},"reference-count":35,"publisher":"Wiley","issue":"38","license":[{"start":{"date-parts":[[2025,7,21]],"date-time":"2025-07-21T00:00:00Z","timestamp":1753056000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,7,21]],"date-time":"2025-07-21T00:00:00Z","timestamp":1753056000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000865","name":"Bill and Melinda Gates Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000865","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advanced Science"],"published-print":{"date-parts":[[2025,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    In 2023 malaria claimed \u2248600000 lives, with 90% of those deaths attributed to the\n                    <jats:italic>Plasmodium falciparum<\/jats:italic>\n                    parasite. This resurgence in mortality emphasizes the necessity of adopting alternative models to accelerate therapeutic development. The Malaria\u2010on\u2010a\u2010Chip model used here incorporated human liver, spleen, and endothelium with\n                    <jats:italic>P. falciparum\u2010<\/jats:italic>\n                    infected blood, and was maintained for 7 days using serum\u2010free medium. This model sustained all stages of the intraerythrocytic life cycle and allowed for organ\u2013organ interaction, providing advantageous preclinical insight into malaria pathophysiology. Chloroquine, lumefantrine, or artesunate were delivered as monotherapies to 3D7 or W2\u2010infected systems. Dose\u2010dependent parasite clearance was observed in both strains for all compounds. Recrudescence occurred in the 3D7\u2010infected model following treatment with chloroquine or lumefantrine, but not artesunate. In W2\u2010infected systems, chloroquine and lumefantrine treatment resulted in parasitemia stabilization by day 7, while artesunate further reduced parasitemia. Population dynamics modeling of pharmacokinetic and pharmacodynamic (PK\/PD) outcomes were utilized to predict human in vivo parameters for efficacy and off\u2010target toxicity using in vitro results.\n                  <\/jats:p>","DOI":"10.1002\/advs.202505206","type":"journal-article","created":{"date-parts":[[2025,7,21]],"date-time":"2025-07-21T10:35:48Z","timestamp":1753094148000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Translation of a Human\u2010Based Malaria\u2010on\u2010a\u2010Chip Phenotypic Disease Model for In Vivo Applications"],"prefix":"10.1002","volume":"12","author":[{"given":"Michael J.","family":"Rupar","sequence":"first","affiliation":[{"name":"Hesperos, Inc.  12501 Research Parkway, Suite 100 Orlando FL 32826 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hannah M.","family":"Hanson","sequence":"additional","affiliation":[{"name":"Hesperos, Inc.  12501 Research 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W.","family":"McAleer","sequence":"additional","affiliation":[{"name":"Hesperos, Inc.  12501 Research Parkway, Suite 100 Orlando FL 32826 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephan","family":"Schmidt","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutics Center for Pharmacometrics and Systems Pharmacology College of Pharmacy University of Florida Orlando Florida 32827 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paola","family":"Favuzza","sequence":"additional","affiliation":[{"name":"Medicines for Malaria Ventures  Rte de Pr\u00e9\u2010Bois 20 Meyrin 1215 Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philip","family":"Lowe","sequence":"additional","affiliation":[{"name":"Medicines for Malaria Ventures  Rte de Pr\u00e9\u2010Bois 20 Meyrin 1215 Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathalie","family":"Gobeau","sequence":"additional","affiliation":[{"name":"Medicines for Malaria Ventures  Rte de Pr\u00e9\u2010Bois 20 Meyrin 1215 Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2608-5001","authenticated-orcid":false,"given":"James J.","family":"Hickman","sequence":"additional","affiliation":[{"name":"Hesperos, Inc.  12501 Research Parkway, Suite 100 Orlando FL 32826 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