{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T16:52:06Z","timestamp":1791823926475,"version":"4.3.4"},"reference-count":33,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T00:00:00Z","timestamp":1767916800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T00:00:00Z","timestamp":1767916800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2022YFD1801105"],"award-info":[{"award-number":["2022YFD1801105"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32172897"],"award-info":[{"award-number":["32172897"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advanced Science"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Pulmonary fibrosis (PF) is the final stage of lung damage, such as chronic obstructive pulmonary disease (COPD), with no effective treatment. Transforming growth factor beta 1 (TGF\u2010\u03b21) is a key protein involved in fibrosis and regulating inflammation. Therefore, targeting components of TGF\u2010\u03b21 is an effective strategy for controlling PF. Artemisia rupestris L, a perennial herb of rupestris belonging to Artemisia, has been prescribed as a treatment for pulmonary inflammation. We investigated the effects and mechanisms of\n                    <jats:italic>Artemisia rupestris<\/jats:italic>\n                    L ethanol extract (EEAR) on PF induced by cigarette smoke (CS) in vitro and in vivo models. In addition, we used Biolayer Interferometry (BLI) and Liquid Chromatograph\u2010Mass Spectrometer (LC\u2010MS) to screen and identify compounds that bind to TGF\u2010\u03b21 in EEAR. We found that EEAR inhibited PF, lung inflammation, and airway obstruction, thereby improving lung injury and blood oxygen levels in COPD. And we identified the active ingredient in EEAR that binds to TGF\u2010\u03b21, rupestonic acid (RA). RA inhibited the TGF\u2010\u03b21\u2010Smad2\/3 signaling pathway by suppressing TGF\u2010\u03b21 ubiquitination and changing its conformation. Furthermore, RA significantly inhibited epithelial mesenchymal transition (EMT) and collagen deposition, thereby treating PF. Based on these findings, we propose that RA might be a promising therapeutic drug candidate for treating PF.\n                  <\/jats:p>","DOI":"10.1002\/advs.202505256","type":"journal-article","created":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T12:30:17Z","timestamp":1767961817000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Rupestonic Acid of\n                    <i>Artemisia Rupestris<\/i>\n                    L. Extract Treats Pulmonary Fibrosis in COPD by Targeting\u00a0TGF\u2010\u03b21"],"prefix":"10.1002","volume":"13","author":[{"given":"Lingfeng","family":"Peng","sequence":"first","affiliation":[{"name":"State Key Laboratory of Veterinary Public Health and Safety China Agricultural University  Beijing China"},{"name":"Chinese Veterinary Medicine Innovation Center College of Veterinary Medicine China Agricultural University  Beijing China"},{"name":"Key Biology Laboratory of Chinese Veterinary Medicine Ministry of Agriculture and Rural Affairs  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lulu","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Veterinary Public Health and Safety China Agricultural University  Beijing China"},{"name":"Chinese Veterinary Medicine Innovation Center College of Veterinary Medicine China Agricultural University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yimeng","family":"Fan","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Veterinary Public Health and Safety China Agricultural University  Beijing China"},{"name":"Chinese Veterinary Medicine Innovation Center College of Veterinary Medicine China Agricultural University  Beijing China"},{"name":"Key Biology Laboratory of Chinese Veterinary Medicine Ministry of Agriculture and Rural Affairs  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sijuan","family":"Huang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Veterinary Public Health and Safety China Agricultural University  Beijing China"},{"name":"Chinese Veterinary Medicine Innovation Center College of Veterinary Medicine China Agricultural University  Beijing China"},{"name":"Key Biology Laboratory of Chinese Veterinary Medicine Ministry of Agriculture and Rural Affairs  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingyu","family":"Zhao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Veterinary Public Health and Safety China Agricultural University  Beijing China"},{"name":"Chinese Veterinary Medicine Innovation Center College of Veterinary Medicine China Agricultural University  Beijing China"},{"name":"Key Biology Laboratory of Chinese Veterinary Medicine Ministry of Agriculture and Rural Affairs  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chao","family":"Han","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Veterinary Public Health and Safety China Agricultural University  Beijing China"},{"name":"Chinese Veterinary Medicine Innovation Center College of Veterinary Medicine China Agricultural University  Beijing China"},{"name":"Key Biology Laboratory of Chinese Veterinary Medicine Ministry of Agriculture and Rural Affairs  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7282-2340","authenticated-orcid":false,"given":"Zhihui","family":"Hao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Veterinary Public Health and Safety China Agricultural University  Beijing China"},{"name":"Chinese Veterinary Medicine Innovation Center College of Veterinary Medicine China Agricultural University  Beijing China"},{"name":"Key Biology Laboratory of Chinese Veterinary Medicine Ministry of Agriculture and Rural Affairs  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,1,9]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI60323"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2213\u20102600(20)30225\u20103"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pharmthera.2018.02.010"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules27092634"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0183720"},{"key":"e_1_2_10_7_1","volume-title":"Pharmacopoeia of the People's Republic of China","year":"1992"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox11030568"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmcl.2014.08.004"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jnatprod.5b00894"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.matbio.2018.01.018"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2020\u20100348ED"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibs.2017.09.002"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioorg.2024.107611"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1474-4422(21)00252-0"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2023.116229"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chest.2017.10.012"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24044004"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24043786"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2023.114472"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392\u2010024\u201001757\u20109"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13020\u2010020\u201000373\u20103"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11882\u2010020\u201000958\u2010w"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2015\u20100391TR"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbi.2018.07.008"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sbi.2023.102737"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox11020181"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1186\/s10020\u2010021\u201000309\u2010z"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1155\/2022\/5595781"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2023.155078"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2024.155564"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202414587"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.apsb.2023.03.011"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jare.2021.04.007"}],"container-title":["Advanced Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/advs.202505256","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/advs.202505256","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/advs.202505256","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T08:08:29Z","timestamp":1770970109000},"score":1,"resource":{"primary":{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/10.1002\/advs.202505256"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1,9]]},"references-count":33,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2026,2]]}},"alternative-id":["10.1002\/advs.202505256"],"URL":"https:\/\/doi.org\/10.1002\/advs.202505256","archive":["Portico"],"relation":{},"ISSN":["2198-3844","2198-3844"],"issn-type":[{"value":"2198-3844","type":"print"},{"value":"2198-3844","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1,9]]},"assertion":[{"value":"2025-03-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-10-13","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-01-09","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e05256"}}