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The components and immunity mechanisms of CRISPR\u2010Cas have been extensively studied, but the regulation of this system in\n                    <jats:italic>Staphylococci<\/jats:italic>\n                    remains unclear. Here, it is shown that the cell\u2010cell communication, known as quorum sensing (QS), inhibits the expression and activity of the type III\u2010A CRISPR\u2010Cas system in\n                    <jats:italic>S. aureus<\/jats:italic>\n                    . The QS regulator, AgrA, directly binds to the promoters of two transcriptional regulators encoding the genes\n                    <jats:italic>sarA<\/jats:italic>\n                    and\n                    <jats:italic>arcR<\/jats:italic>\n                    to inhibit their expression. However, both SarA and ArcR act as positive regulators that promote the transcription of\n                    <jats:italic>cas<\/jats:italic>\n                    genes by directly binding to a novel promoter P\n                    <jats:italic>cas<\/jats:italic>\n                    . Furthermore, the P\n                    <jats:italic>cas<\/jats:italic>\n                    of 300\u00a0bp located in\n                    <jats:italic>cas1<\/jats:italic>\n                    displays as a critical regulatory node to initiate the transcription of\n                    <jats:italic>cas10<\/jats:italic>\n                    and\n                    <jats:italic>csm3<\/jats:italic>\n                    . Our data reveal a new regulatory mechanism for QS\u2010mediated repression of the Type III\u2010A CRISPR\u2010Cas system, which may allow\n                    <jats:italic>S. aureus<\/jats:italic>\n                    to acquire foreign genetic elements encoding antibiotic resistance or virulence factors specifically at high cell density.\n                  <\/jats:p>","DOI":"10.1002\/advs.202506049","type":"journal-article","created":{"date-parts":[[2025,7,11]],"date-time":"2025-07-11T09:43:30Z","timestamp":1752227010000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Quorum Sensing Inhibits Type III\u2010A CRISPR\u2010Cas System Activity Through Repressing Positive Regulators SarA and ArcR in\n                    <i>Staphylococcus Aureus<\/i>"],"prefix":"10.1002","volume":"12","author":[{"given":"Yang","family":"Li","sequence":"first","affiliation":[{"name":"Jiangsu Key Lab of Zoonosis\/Jiangsu 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