{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T02:44:18Z","timestamp":1785897858885,"version":"3.56.0"},"reference-count":72,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advanced Science"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The discovery of plant biostimulants (PBs) in sewage sludge offers a promising avenue for biosolids valorization. Here, the study investigates how two mineral additives, including 2\u2010line ferrihydrite (a disordered iron oxide) and disordered birnessite (a manganese oxide), modulate microbial activity and molecular pathways to enhance PB production during aerobic sludge composting. Application of 2\u2010line ferrihydrite significantly promotes the synthesis of growth\u2010promoting PBs, including arginine, valine, decanoic acid, and indoleacetic acid (IAA), while disordered birnessite primarily enhances resistance\u2010related PBs, such as decanoic acid, L\u2010pyroglutamate, and trans\u2010aconitic acid. In pot trials, composted biosolids amended with 2\u2010line ferrihydrite significantly improve plant biomass and leaf area compared to mineral\u2010free and birnessite treatments. Metagenomic profiling reveals that PB biosynthesis is dominated by members of the phyla Pseudomonadota and Actinomycetota, with temporal niche partitioning across the thermophilic and maturation stages. 2\u2010line ferrihydrite enhances the abundance of critical biosynthetic genes (e.g.,\n                    <jats:italic>trpA\/C\/D\/E\/F<\/jats:italic>\n                    ), particularly within taxa such as\n                    <jats:italic>Xanthomonadaceae<\/jats:italic>\n                    ,\n                    <jats:italic>Sphingomonadaceae, and Streptosporangiaceae<\/jats:italic>\n                    . Additionally, genes involved in IAA and indole biosysnthesis (\n                    <jats:italic>ALDH<\/jats:italic>\n                    ,\n                    <jats:italic>DDC<\/jats:italic>\n                    , and\n                    <jats:italic>tnaA<\/jats:italic>\n                    ) are enriched, supporting enhanced tryptophan\u2010to\u2010IAA conversion. This study provides a mechanistic link between iron oxide\u2010mediated microbial modulation and PB production in composted biosolids, offering a sustainable approach for upgrading waste into high\u2010value agricultural inputs.\n                  <\/jats:p>","DOI":"10.1002\/advs.202506502","type":"journal-article","created":{"date-parts":[[2025,12,2]],"date-time":"2025-12-02T07:01:30Z","timestamp":1764658890000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["2\u2010line Ferrihydrite Enhance Microbial Synthesis of Plant Biostimulants in Composted Biosolid by Regulating Phyla\n                    <i>Pseudomonadota<\/i>\n                    and\n                    <i>Actinomycetota<\/i>"],"prefix":"10.1002","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2624-2994","authenticated-orcid":false,"given":"Yu","family":"Zhang","sequence":"first","affiliation":[{"name":"School of Environmental Studies China University of Geosciences  Wuhan Hubei 430074 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziwei","family":"Zhang","sequence":"additional","affiliation":[{"name":"National Engineering Research Center for Industrial Wastewater Detoxication and Resource Recovery Research Center for Eco\u2010Environmental Sciences Chinese Academy of Sciences  Beijing 100085 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zexu","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Environmental Studies China University of Geosciences  Wuhan Hubei 430074 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Boyuan","family":"Yang","sequence":"additional","affiliation":[{"name":"School of Environmental Studies China University of Geosciences  Wuhan Hubei 430074 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siying","family":"Cai","sequence":"additional","affiliation":[{"name":"School of Environmental Studies China University of Geosciences  Wuhan Hubei 430074 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Environmental Studies China University of Geosciences  Wuhan Hubei 430074 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianhua","family":"Guo","sequence":"additional","affiliation":[{"name":"Australia Centre for Water and Environmental Biotechnology (ACWEB, formerly AWMC) The University of Queensland  St Lucia 4067 Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5654-6270","authenticated-orcid":false,"given":"Weijun","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Environmental Studies China University of Geosciences  Wuhan Hubei 430074 China"},{"name":"National Engineering Research Center for Industrial Wastewater Detoxication and Resource Recovery Research Center for Eco\u2010Environmental Sciences Chinese Academy of Sciences  Beijing 100085 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,12,1]]},"reference":[{"key":"e_1_2_9_1_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.watres.2020.115912"},{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s44284-023-00021-5"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/en15155633"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1021\/acssuschemeng.0c00015"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.2166\/wst.1997.0518"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.envint.2020.106093"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/pr11030731"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jclepro.2019.01.148"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biortech.2016.05.065"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1021\/acssuschemeng.0c06899"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2022.158712"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biortech.2017.09.193"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.wasman.2009.03.016"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.watres.2024.121446"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jclepro.2022.132953"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2020.141125"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.2138\/am.2005.1591"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jenvman.2023.119952"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clay.2009.08.017"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/s43016-022-00597-6"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cej.2024.153232"},{"key":"e_1_2_9_22_1","volume":"11","author":"Li N.","year":"2021","journal-title":"Front. Plant Sci."},{"key":"e_1_2_9_23_1","doi-asserted-by":"crossref","unstructured":"Y.Ichihashi Y.Date A.Shino T.Shimizu A.Shibata K.Kumaishi F.Funahashi K.Wakayama K.Yamazaki A.Umezawa T.Sato M.Kobayashi M.Kamimura M.Kusano F.\u2010S.Che M.O`Brien K.Tanoi M.Hayashi R.Nakamura K.Shirasu J.Kikuchi N.Nihei PNAS2020 117 14552.","DOI":"10.1073\/pnas.1917259117"},{"key":"e_1_2_9_24_1","volume-title":"Iron oxides in the laboratory: preparation and characterization","author":"Schwertmann U.","year":"2008"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0016-7037(03)00217-5"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biortech.2011.01.083"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-8524(99)00018-8"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-2697(02)00704-2"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/0043-1354(95)00323-1"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2019.134360"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhazmat.2025.137183"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1021\/es5002105"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-44422-5"},{"key":"e_1_2_9_34_1","unstructured":"F.C\u00e1mara\u2010Martos A.De Haro\u2010Bail\u00f3n M. E.Cartea S.Obregon\u2010Cano F. R.Badenes\u2010P\u00e9rez (Eds.:A. K. M. A.Islam M. A.Hossain A. K. M. M.Islam) IntechOpen London2021."},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1180\/claymin.1993.028.2.02"},{"key":"e_1_2_9_36_1","first-page":"477","volume":"75","author":"Post J. E.","year":"1990","journal-title":"Am. Mineral."},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-2911(04)49005-5"},{"key":"e_1_2_9_38_1","unstructured":"D. R.Lovley in (Ed.:D. R.Lovley) American Society for Microbiology Washington DC2000."},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.watres.2020.116441"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biortech.2023.129795"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biortech.2021.126530"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biortech.2018.08.079"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biortech.2019.122596"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1029\/2001GL013839"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.est.7b04379"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.3390\/su17167203"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemosphere.2023.138414"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12932-015-0026-0"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.3390\/metabo13080884"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1111\/1758-2229.12980"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12013-013-9750-1"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a001438"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2015.08.001"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2020.00891"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.3390\/plants12081596"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.envexpbot.2018.10.034"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymben.2023.06.007"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.3390\/plants12071492"},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.107.107953"},{"key":"e_1_2_9_60_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2015.00534"},{"key":"e_1_2_9_61_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2020.571244"},{"key":"e_1_2_9_62_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2012.00404"},{"key":"e_1_2_9_63_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01517-16"},{"key":"e_1_2_9_64_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biortech.2022.128544"},{"key":"e_1_2_9_65_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.est.4c06885"},{"key":"e_1_2_9_66_1","doi-asserted-by":"publisher","DOI":"10.1007\/s44154-024-00175-9"},{"key":"e_1_2_9_67_1","doi-asserted-by":"publisher","DOI":"10.2215\/CJN.07730713"},{"key":"e_1_2_9_68_1","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2022.878686"},{"key":"e_1_2_9_69_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.envres.2023.116460"},{"key":"e_1_2_9_70_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10295-018-2064-y"},{"key":"e_1_2_9_71_1","doi-asserted-by":"publisher","DOI":"10.1093\/femsre\/fux049"},{"key":"e_1_2_9_72_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13068-023-02447-4"}],"container-title":["Advanced Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/advs.202506502","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/advs.202506502","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/advs.202506502","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T08:08:12Z","timestamp":1770970092000},"score":1,"resource":{"primary":{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/10.1002\/advs.202506502"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12,1]]},"references-count":72,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2026,2]]}},"alternative-id":["10.1002\/advs.202506502"],"URL":"https:\/\/doi.org\/10.1002\/advs.202506502","archive":["Portico"],"relation":{},"ISSN":["2198-3844","2198-3844"],"issn-type":[{"value":"2198-3844","type":"print"},{"value":"2198-3844","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,12,1]]},"assertion":[{"value":"2025-04-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-12-01","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e06502"}}