{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T14:45:13Z","timestamp":1787496313530,"version":"build-2736575974"},"reference-count":77,"publisher":"Wiley","issue":"46","license":[{"start":{"date-parts":[[2025,9,29]],"date-time":"2025-09-29T00:00:00Z","timestamp":1759104000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advanced Science"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The tongue is essential for swallowing, taste perception, and mechanosensation. The anterior and posterior parts of the tongue have region\u2010specific developmental origins and are maintained by adult epithelial stem\/progenitor cells. In vitro models that can be used to investigate anterior tongue biology have been lacking. Here, a protocol is developed to generate a long\u2010term expanding organoid model from the adult mouse dorsal anterior tongue. Anterior tongue organoids consist of\n                    <jats:italic>Lgr6<\/jats:italic>\n                    + cells,\n                    <jats:italic>Sox2<\/jats:italic>\n                    + stem\/progenitor cells, and\n                    <jats:italic>Hoxc13<\/jats:italic>\n                    + filiform papillae progenitor cells. Furthermore, anterior tongue organoids share region\u2010specific transcriptomic profiles, gene regulatory networks, and signaling pathways with anterior tongue tissue. Anterior tongue organoids can be differentiated into various epithelial cell types, including Merkel\u2010like cells, keratinocytes, and taste bud cells. Gene regulatory network analysis reveals transcriptional programs associated with\n                    <jats:italic>Krt8<\/jats:italic>\n                    + cell and\n                    <jats:italic>Krt23+\/Sbsn+<\/jats:italic>\n                    keratinocyte differentiation in the organoids. Together, this study provides an in vitro model of mouse dorsal tongue epithelium.\n                  <\/jats:p>","DOI":"10.1002\/advs.202506738","type":"journal-article","created":{"date-parts":[[2025,9,29]],"date-time":"2025-09-29T13:39:19Z","timestamp":1759153159000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Organoid Modeling of Mouse Anterior Tongue Epithelium Reveals Regional and Cellular Identities"],"prefix":"10.1002","volume":"12","author":[{"given":"Seok\u2010Young","family":"Kim","sequence":"first","affiliation":[{"name":"Princess M\u00e1xima Center for Pediatric Oncology  Utrecht 3584 CS The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurens H. G.","family":"Verweij","sequence":"additional","affiliation":[{"name":"Princess M\u00e1xima Center for Pediatric Oncology  Utrecht 3584 CS The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Lin","sequence":"additional","affiliation":[{"name":"Princess M\u00e1xima Center for Pediatric Oncology  Utrecht 3584 CS The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johan H.","family":"van Es","sequence":"additional","affiliation":[{"name":"Hubrecht Institute Royal Netherlands Academy of Arts and Sciences (KNAW)  Utrecht 3508 AD The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jay","family":"Slack","sequence":"additional","affiliation":[{"name":"Department of Science + Technology Givaudan Flavors Corp  Cincinnati OH 45216 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chris","family":"Winkel","sequence":"additional","affiliation":[{"name":"Givaudan Nederland Flavors  Naarden 1411 GP The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tito","family":"Candelli","sequence":"additional","affiliation":[{"name":"Princess M\u00e1xima Center for Pediatric Oncology  Utrecht 3584 CS The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philip","family":"Lijnzaad","sequence":"additional","affiliation":[{"name":"Princess M\u00e1xima Center for Pediatric Oncology  Utrecht 3584 CS The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thanasis","family":"Margaritis","sequence":"additional","affiliation":[{"name":"Princess M\u00e1xima Center for Pediatric Oncology  Utrecht 3584 CS The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerben E.","family":"Breimer","sequence":"additional","affiliation":[{"name":"Department of Pathology University Medical Center Utrecht  Utrecht 3508 GA The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karin","family":"Sanders","sequence":"additional","affiliation":[{"name":"Princess M\u00e1xima Center for Pediatric Oncology  Utrecht 3584 CS The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc","family":"van de Wetering","sequence":"additional","affiliation":[{"name":"Princess M\u00e1xima Center for Pediatric Oncology  Utrecht 3584 CS The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8566-839X","authenticated-orcid":false,"given":"Hans","family":"Clevers","sequence":"additional","affiliation":[{"name":"Princess M\u00e1xima Center for Pediatric Oncology  Utrecht 3584 CS The Netherlands"},{"name":"Hubrecht Institute Royal Netherlands Academy of Arts and Sciences (KNAW)  Utrecht 3508 AD The Netherlands"},{"name":"Roche Pharmaceutical Research and Early Development (pRED) of F. Hoffmann\u2010La Roche Ltd  CH\u20104070 Basel Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,9,29]]},"reference":[{"key":"e_1_2_8_1_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00012.2022"},{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.2008-0611"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/stem.1338"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1409064111"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-28195-2"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-021-03561-1"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature07935"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.23804"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1715272116"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep17185"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-022-04242-0"},{"key":"e_1_2_8_12_1","doi-asserted-by":"crossref","unstructured":"L.Verweij S.Kim D.Laskaris L.Lin G.vanSon F.Ringnalda C.Winkel J.Slack K.Sanders J.vanRheenen M.van deWetering Tripotent Lgr5 stem cells in the posterior tongue generate non\u2010taste lingual taste bud and salivary gland cells2024 https:\/\/www.researchsquare.com\/article\/rs\u20104899576\/v1.","DOI":"10.21203\/rs.3.rs-4899576\/v1"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep03224"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.182542899"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2011.07.050"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.107631"},{"key":"e_1_2_8_17_1","first-page":"3054","volume":"144","author":"Castillo\u2010Azofeifa D.","year":"2017","journal-title":"Development"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1006442"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2013.07.022"},{"key":"e_1_2_8_20_1","first-page":"E200","volume":"115","author":"Lu W.\u2010J.","year":"2018","journal-title":"Proc. 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