{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:26:32Z","timestamp":1786976792580,"version":"build-2736575974"},"reference-count":82,"publisher":"Wiley","issue":"20","license":[{"start":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T00:00:00Z","timestamp":1772064000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T00:00:00Z","timestamp":1772064000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advanced Science"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Regenerative medicine offers a promising approach to treat brain\u00a0injuries, yet challenges persist in promoting neuronal survival and integration. Recent studies demonstrate that human cells implanted into rodent brains can exhibit plasticity, integrate into neural circuits and alleviate functional deficits. However, integration is often poor, with inadequate vascularization, and insufficient support cells such as astrocytes. Astrocytes play a crucial role in neuronal development and recovery by releasing growth factors, facilitating synaptogenesis, and promoting blood vessel formation. This study investigated human neuronal progenitor cells cultured alone or cultured with mouse astrocytes and formed into 3D constructs using microfluidics. Co\u2010cultures exhibited enhanced neuronal maturation, viability, and density. Following implantation into mouse brains, co\u2010cultures reduced lesion size, increased axonal growth, and improved astrocyte coupling to blood vessels within the graft. Additionally, we show that NPCs and co\u2010cultures increased astrocyte size in implants. Deconvolved high\u2010resolution microscopy identified synapses and optogenetics showed functional connections between the host and implants. These findings underscore the essential role of astrocytes in enhancing neuronal tissue integration and advancing brain injury treatments.<\/jats:p>","DOI":"10.1002\/advs.202507423","type":"journal-article","created":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T08:18:00Z","timestamp":1772957880000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Astrocyte Enrichment of 3D Cortical Constructs Enhances Brain Repair"],"prefix":"10.1002","volume":"13","author":[{"given":"Elisa M.","family":"Cruz","sequence":"first","affiliation":[{"name":"Department of Physiology, Anatomy and Genetics University of Oxford  Oxford UK"},{"name":"Aston Institute for Membrane Excellence and School of Biosciences Aston University  Birmingham UK"},{"name":"BHF Oxford Centre of Research Excellence University of Oxford  Oxford UK"},{"name":"Kavli Institute for NanoScience Discovery University of Oxford  Oxford UK"},{"name":"British Heart Foundation (BHF) \u2010 UK Dementia Research Institute (UK DRI) Centre for Vascular Dementia Research at the University of Oxford  Oxford UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luana C.","family":"Soares","sequence":"additional","affiliation":[{"name":"Department of Physiology, Anatomy and Genetics University of Oxford  Oxford UK"},{"name":"BHF Oxford Centre of Research Excellence University of Oxford  Oxford UK"},{"name":"Kavli Institute for NanoScience Discovery University of Oxford  Oxford UK"},{"name":"British Heart Foundation (BHF) \u2010 UK Dementia Research Institute (UK DRI) Centre for Vascular Dementia Research at the University of Oxford  Oxford UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gretchen","family":"Greene","sequence":"additional","affiliation":[{"name":"Department of Physiology, Anatomy and Genetics University of Oxford  Oxford UK"},{"name":"National Institute of Neurological Disorders NIH  Bethesda MD USA"},{"name":"Faculty of Medicine Alexandria University  Alexandria Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernando","family":"Messore","sequence":"additional","affiliation":[{"name":"Department of Physiology, Anatomy and Genetics University of Oxford  Oxford UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammed","family":"Abuelem","sequence":"additional","affiliation":[{"name":"Department of Physiology, Anatomy and Genetics University of Oxford  Oxford UK"},{"name":"National Institute of Mental Health NIH  Bethesda MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingyu","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Chemistry University of Oxford  Oxford UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caroline","family":"Andersen","sequence":"additional","affiliation":[{"name":"Department of Physiology, Anatomy and Genetics University of Oxford  Oxford UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mirriam","family":"Domocos","sequence":"additional","affiliation":[{"name":"Department of Physiology, Anatomy and Genetics University of Oxford  Oxford UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisa","family":"Vitiello","sequence":"additional","affiliation":[{"name":"Department of Biochemistry University of Oxford  Oxford UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rabeah Abdul","family":"Razak","sequence":"additional","affiliation":[{"name":"Department of Physiology, Anatomy and Genetics University of Oxford  Oxford UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marlene","family":"Lawston","sequence":"additional","affiliation":[{"name":"Department of Physiology, Anatomy and Genetics University of Oxford  Oxford UK"},{"name":"National Institute of Mental Health NIH  Bethesda MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriel","family":"Moser","sequence":"additional","affiliation":[{"name":"Department of Chemistry University of Oxford  Oxford UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Talia","family":"Vasaturo\u2010Kolodner","sequence":"additional","affiliation":[{"name":"Department of Physiology, Anatomy and Genetics University of Oxford  Oxford UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mona","family":"Barkat","sequence":"additional","affiliation":[{"name":"Department of Physiology, Anatomy and Genetics University of Oxford  Oxford UK"},{"name":"Faculty of Medicine Alexandria University  Alexandria Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roslyn M.","family":"Bill","sequence":"additional","affiliation":[{"name":"Department of Physiology, Anatomy and Genetics University of Oxford  Oxford UK"},{"name":"Aston Institute for Membrane Excellence and School of Biosciences Aston University  Birmingham UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edward","family":"Mann","sequence":"additional","affiliation":[{"name":"Department of Physiology, Anatomy and Genetics University of Oxford  Oxford UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linna","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Chemistry University of Oxford  Oxford UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mootaz M.","family":"Salman","sequence":"additional","affiliation":[{"name":"Department of Physiology, Anatomy and Genetics University of Oxford  Oxford UK"},{"name":"BHF Oxford Centre of Research Excellence University of Oxford  Oxford UK"},{"name":"Kavli Institute for NanoScience Discovery University of Oxford  Oxford UK"},{"name":"British Heart Foundation (BHF) \u2010 UK Dementia Research Institute (UK DRI) Centre for Vascular Dementia Research at the University of Oxford  Oxford UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hagan","family":"Bayley","sequence":"additional","affiliation":[{"name":"Department of Chemistry University of Oxford  Oxford UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zolt\u00e1n","family":"Moln\u00e1r","sequence":"additional","affiliation":[{"name":"Department of Physiology, Anatomy and Genetics University of Oxford  Oxford UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2931-0673","authenticated-orcid":false,"given":"Francis G.","family":"Szele","sequence":"additional","affiliation":[{"name":"Department of Physiology, Anatomy and Genetics University of Oxford  Oxford UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,2,26]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.conb.2014.03.005"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrn3484"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1177\/08830738030180090901"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2015.02.001"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn1970"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/cercor\/10.5.443"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-3806(94)90203-8"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2018.04.094"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2012.12.011"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature07287"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-024-02157-8"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0046628"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1177\/1087057117696795"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmolb.2020.00033"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.201909146"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.202002183"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41596-018-0032-7"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-06358-x"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-41356-w"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.4155\/bio.12.133"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/micronano.2015.45"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12575-023-00194-2"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-53414-9"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41593-018-0175-4"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2015.00070"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-025-09010-1"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nbd.2013.11.003"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2019.00822"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-024-50190-7"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.201901152"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-025-02249-0"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/321209"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nbd.2019.104672"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.3390\/biomedicines12081693"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2424-23.2024"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2012.116"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.2174\/1389450117666160101120703"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.4707-08.2009"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnana.2023.1130729"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00429-017-1383-5"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13024-021-00487-8"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neulet.2013.12.030"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2019.08.009"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn.3041"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3940(02)00611-0"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.15-06-04429.1995"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnins.2024.1425525"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.20445"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2023.1094503"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.3389\/fneur.2022.835597"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2007.04.019"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.3390\/brainsci10120909"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2021.109048"},{"key":"e_1_2_9_55_1","author":"Mansour A. A.","year":"2018","journal-title":"Nature Biotechnology"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00011.2013"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.1186\/1742-2094-9-279"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.20039"},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109.010090"},{"key":"e_1_2_9_60_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-008-0658-9"},{"key":"e_1_2_9_61_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnins.2022.966772"},{"key":"e_1_2_9_62_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41380-020-00910-4"},{"key":"e_1_2_9_63_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-022-05277-w"},{"key":"e_1_2_9_64_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2017.07.035"},{"key":"e_1_2_9_65_1","doi-asserted-by":"publisher","DOI":"10.1523\/ENEURO.0148-24.2024"},{"key":"e_1_2_9_66_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2018.04.066"},{"key":"e_1_2_9_67_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stemcr.2021.07.001"},{"key":"e_1_2_9_68_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2012.12.015"},{"key":"e_1_2_9_69_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tins.2006.08.004"},{"key":"e_1_2_9_70_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.40070"},{"issue":"2","key":"e_1_2_9_71_1","first-page":"2908","article-title":"Appearance of \u00df\u2010dystroglycan precedes the formation of glio\u2010vascular end\u2010feet in developing rat brain","volume":"62","author":"Kalman M.","year":"2018","journal-title":"European Journal of Histochemistry"},{"issue":"3","key":"e_1_2_9_72_1","first-page":"234","article-title":"Traumatic brain injury in infants and toddlers, 0\u20133 years old","volume":"4","author":"Ciurea A. V.","year":"2011","journal-title":"Journal of medicine and life"},{"key":"e_1_2_9_73_1","doi-asserted-by":"publisher","DOI":"10.1038\/pr.2011.64"},{"key":"e_1_2_9_74_1","doi-asserted-by":"publisher","DOI":"10.1002\/wsbm.1557"},{"key":"e_1_2_9_75_1","doi-asserted-by":"publisher","DOI":"10.2176\/nmc.ra.2016-0191"},{"key":"e_1_2_9_76_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12974-022-02390-5"},{"key":"e_1_2_9_77_1","doi-asserted-by":"publisher","DOI":"10.3791\/50079"},{"key":"e_1_2_9_78_1","doi-asserted-by":"publisher","DOI":"10.1038\/tp.2013.71"},{"key":"e_1_2_9_79_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2017.020"},{"key":"e_1_2_9_80_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jneumeth.2016.09.001"},{"key":"e_1_2_9_81_1","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2022.958876"},{"key":"e_1_2_9_82_1","doi-asserted-by":"publisher","DOI":"10.1039\/C5LC00133A"},{"key":"e_1_2_9_83_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnins.2022.829884"}],"container-title":["Advanced Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/advs.202507423","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/advs.202507423","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/advs.202507423","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,10]],"date-time":"2026-04-10T11:09:37Z","timestamp":1775819377000},"score":1,"resource":{"primary":{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/10.1002\/advs.202507423"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2,26]]},"references-count":82,"journal-issue":{"issue":"20","published-print":{"date-parts":[[2026,4]]}},"alternative-id":["10.1002\/advs.202507423"],"URL":"https:\/\/doi.org\/10.1002\/advs.202507423","archive":["Portico"],"relation":{},"ISSN":["2198-3844","2198-3844"],"issn-type":[{"value":"2198-3844","type":"print"},{"value":"2198-3844","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,2,26]]},"assertion":[{"value":"2025-04-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-01-06","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-02-26","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e07423"}}