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During morphogenesis, cellular self\u2010organization and matrix development are strongly influenced by the mechanical constraints provided by surrounding tissues, suggesting that such biophysical cues should be integrated into bioprinting strategies to engineer more biomimetic grafts. Here, we introduce a novel bioprinting platform that spatially patterns mesenchymal stem\/stromal cell (MSC)\u2010derived microtissues into mechanically tunable support baths. By modulating the bath's mechanical properties, we can precisely control the physical constraints applied post\u2010printing, directing both filament geometry and cellular behavior. Support bath stiffness regulated mechano\u2010sensitive gene expression and microtissue phenotype, with softer matrices favoring chondrogenesis and stiffer environments promoting (myo)fibrogenic differentiation. In addition, the physical properties of the non\u2010degradable support bath modulated microtissue fusion and extracellular matrix organization, with increased collagen fiber alignment in stiffer baths. Leveraging these findings, it was possible to engineer either articular cartilage, meniscus, or ligament grafts with user\u2010defined collagen architectures by simply varying the physical properties of the support bath. This platform establishes a foundation for bioprinting structurally anisotropic and phenotypically distinct constructs, thereby enabling the scalable engineering of a range of different musculoskeletal tissues.<\/jats:p>","DOI":"10.1002\/advs.202509313","type":"journal-article","created":{"date-parts":[[2026,2,6]],"date-time":"2026-02-06T12:46:50Z","timestamp":1770382010000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Bioprinting of Microtissues Within Mechanically Tunable Support Baths to Engineer Anisotropic Musculoskeletal Tissues"],"prefix":"10.1002","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2093-8923","authenticated-orcid":false,"given":"Francesca D.","family":"Spagnuolo","sequence":"first","affiliation":[{"name":"Trinity Centre for Biomedical Engineering Trinity Biomedical Sciences Institute Trinity College Dublin  Dublin Ireland"},{"name":"Department of Mechanical Manufacturing and 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