{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T17:11:12Z","timestamp":1790442672553,"version":"4.1.0"},"reference-count":42,"publisher":"Wiley","issue":"47","license":[{"start":{"date-parts":[[2025,9,26]],"date-time":"2025-09-26T00:00:00Z","timestamp":1758844800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,9,26]],"date-time":"2025-09-26T00:00:00Z","timestamp":1758844800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2021YFA0910100"],"award-info":[{"award-number":["2021YFA0910100"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22474074"],"award-info":[{"award-number":["22474074"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advanced Science"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Persisting as a major global health disparity, HPV16\/18\u2010associated cervical carcinogenesis continues to disproportionately affect populations in resource\u2010limited regions with compromised screening infrastructure. While molecular detection of E6\/E7 oncogenic transcripts surpasses conventional cytology in clinical specificity, current nucleic acid amplification platforms remain impeded by technical complexity, prolonged turnaround times (&gt;4 h), and substantial per\u2010test costs (&gt;$50) that hinder scale\u2010up in low\u2010income settings. Here, RotEx\u2010LAMP\u2010LFA is presented: an innovative point\u2010of\u2010care system integrating microfluidic nucleic acid extraction, rapid isothermal amplification (30 min at 63\u00a0\u00b0C), and combined lateral flow detection in a single disposable cartridge. Validated with 69 clinical specimens (19 histology\u2010confirmed SCC, 14 HPV+ precancerous lesions, 15 HPV+ infections, and 21 healthy controls), this sample\u2010to\u2010answer platform achieved 94.74% sensitivity (18\/19; 95% CI: 84.72\u2013100%) for invasive carcinoma detection with 100% (36\/36; 95% CI: 90.44\u201099.93%) specificity for non\u2010cancer. In addition, a subset of samples is collected through paired self\u2010sampling to validate consistency with clinician\u2010collected specimens, indicating that this innovative platform shows promise for community\u2010level molecular stratification of persistent and progressive HPV infections. It paves the way for accessible, patient\u2010centered cervical cancer risk reporting, with the potential to reduce psychological burden on patients.<\/jats:p>","DOI":"10.1002\/advs.202509468","type":"journal-article","created":{"date-parts":[[2025,9,26]],"date-time":"2025-09-26T11:45:17Z","timestamp":1758887117000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["A Field\u2010Deployable RotEx\u2010LAMP\u2010LFA Platform for Molecular Triage of HPV\u2010Driven Oncogenesis"],"prefix":"10.1002","volume":"12","author":[{"given":"Yuan","family":"Gao","sequence":"first","affiliation":[{"name":"Institute of Molecular Medicine (IMM) Renji Hospital, School of Medicine Shanghai Jiao Tong University  Shanghai 200240 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiang","family":"Wen","sequence":"additional","affiliation":[{"name":"Hangzhou Institute of Medicine (HIM) Zhejiang Cancer Hospital Chinese Academy of Sciences  Hangzhou Zhejiang 310022 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siyuan","family":"Qiao","sequence":"additional","affiliation":[{"name":"Institute of Molecular Medicine (IMM) Renji Hospital, School of Medicine Shanghai Jiao Tong University  Shanghai 200240 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linyi","family":"Deng","sequence":"additional","affiliation":[{"name":"Institute of Molecular Medicine (IMM) Renji Hospital, School of Medicine Shanghai Jiao Tong University  Shanghai 200240 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keyue","family":"Li","sequence":"additional","affiliation":[{"name":"Institute of Molecular Medicine (IMM) Renji Hospital, School of Medicine Shanghai Jiao Tong University  Shanghai 200240 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhuyan","family":"Shao","sequence":"additional","affiliation":[{"name":"Hangzhou Institute of Medicine (HIM) Zhejiang Cancer Hospital Chinese Academy of Sciences  Hangzhou Zhejiang 310022 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaonan","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Forensic Medicine Shanxi Medical University  Taiyuan Shanxi 030001 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Zhu","sequence":"additional","affiliation":[{"name":"Hangzhou Institute of Medicine (HIM) Zhejiang Cancer Hospital Chinese Academy of Sciences  Hangzhou Zhejiang 310022 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chao","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute of Molecular Medicine (IMM) Renji Hospital, School of Medicine Shanghai Jiao Tong University  Shanghai 200240 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0804-2964","authenticated-orcid":false,"given":"Da","family":"Han","sequence":"additional","affiliation":[{"name":"Institute of Molecular Medicine (IMM) Renji Hospital, School of Medicine Shanghai Jiao Tong University  Shanghai 200240 China"},{"name":"Hangzhou Institute of Medicine (HIM) Zhejiang Cancer Hospital Chinese Academy of Sciences  Hangzhou Zhejiang 310022 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weihong","family":"Tan","sequence":"additional","affiliation":[{"name":"Institute of Molecular Medicine (IMM) Renji Hospital, School of Medicine Shanghai Jiao Tong University  Shanghai 200240 China"},{"name":"Hangzhou Institute of Medicine (HIM) Zhejiang Cancer Hospital Chinese Academy of Sciences  Hangzhou Zhejiang 310022 China"},{"name":"Molecular Science and Biomedicine Laboratory (MBL) State Key Laboratory of Chemo\/Biosensing and Chemometrics College of Chemistry and Chemical Engineering College of Biology Aptamer Engineering Center of Hunan Province Hunan University  Changsha Hunan 410082 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,9,26]]},"reference":[{"key":"e_1_2_8_1_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21834"},{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj-2022-070115"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41571-024-00977-w"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41571-024-00904-z"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lanepe.2024.101157"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(24)01097-3"},{"key":"e_1_2_8_7_1","first-page":"547","volume":"330","author":"Perkins R. B.","year":"2023","journal-title":"C. C. S.: A. Review, JAMA"},{"key":"e_1_2_8_8_1","first-page":"184","volume":"69","author":"Smith R. A.","year":"2019","journal-title":"CA A Cancer J Clinicians"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21696"},{"key":"e_1_2_8_10_1","first-page":"2019","volume":"321","author":"Sawaya G. F.","year":"2018","journal-title":"JAMA, J. Am. Med. Assoc."},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamaoncol.2020.1460"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-021-00617-5"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1097\/SLA.0b013e318187aeca"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMcp2108502"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1136\/ijgc-2020-001285"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj-2022-070135"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmed.2024.05.009"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.2192"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(22)00294-7"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2468-2667(23)00076-2"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2021.04.031"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2015.04.015"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2018.05.025"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202406367"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2023.115968"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.abn4768"},{"key":"e_1_2_8_27_1","volume-title":"Target Product Profiles for Human Papillomavirus Screening Tests to Detect Cervical Pre\u2010cancer and Cancer","author":"World Health Organization","year":"2024"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.1004494"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trac.2025.118275"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1093\/jambio\/lxaf070"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.70219"},{"key":"e_1_2_8_32_1","first-page":"1744","author":"Guo Y.","year":"2025","journal-title":"Insect Science"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0321794"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.0c05059"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1039\/D4AY01921K"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21660"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(18)32470-X"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1002\/cac2.12629"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.9b02554"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.adq2899"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0020183"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMc2026172"}],"container-title":["Advanced Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/advs.202509468","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/advs.202509468","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/advs.202509468","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T14:30:19Z","timestamp":1789655419000},"score":1,"resource":{"primary":{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/10.1002\/advs.202509468"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,9,26]]},"references-count":42,"journal-issue":{"issue":"47","published-print":{"date-parts":[[2025,12]]}},"alternative-id":["10.1002\/advs.202509468"],"URL":"https:\/\/doi.org\/10.1002\/advs.202509468","archive":["Portico"],"relation":{},"ISSN":["2198-3844","2198-3844"],"issn-type":[{"value":"2198-3844","type":"print"},{"value":"2198-3844","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,9,26]]},"assertion":[{"value":"2025-05-24","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-09-26","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e09468"}}