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This study aims to develop a preclinical MA model to investigate the dynamic interplay between craniofacial structural defects and neurobehavioral dysfunction. Longitudinal behavioral phenotyping including von Frey filaments test as well as open field and elevated plus maze tests reveals progressive sensory hypersensitivity and anxiety\u2010like behaviors, with computational ethology revealing subtle but consistent alterations in the naturalistic behaviors. Whole\u2010brain activity mapping reveal hyperactivation in the anterior cingulate cortex (ACC), whereas multi\u2010omics profiling reveal cell type\u2010specific transcriptional changes and synaptic reorganization within this region. Functional investigation including sparse labeling, electrophysiology, western blotting, and chemogenetics demonstrate that the ACC modulation regulated both pain and anxiety. These findings establish a causal association between structural craniofacial defects and maladaptive neural circuit remodeling, with the ACC emerging as a critical therapeutic target. 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