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Although stem cell therapy has demonstrated efficacy in preclinical and clinical studies, it faces challenges such as low cell survival (&lt;5%) and uncontrolled glial differentiation. This study aims to develop a 3D\u2010bioprinted neural patch to enhance stem cell therapy for AD. The hypothesis is that a supportive bioengineered microenvironment would improve cell integration and neuronal differentiation, leading to functional recovery. A tri\u2010component bioink (gelatin\/alginate\/fibrinogen) is created with tunable printability, biocompatibility, and biodegradation, establishing functional transplantation microenvironments for a 3D\u2010printed human induced pluripotent stem cell (hiPSC)\u2010derived neural progenitor cell (NPC) construct as a hippocampal patch. The system (TTBT) maintains NPC survival and promotes neuronal differentiation, neurite development, and calcium signaling in vitro. In AD\u2010like rats, these constructs improved cell retention (3.41\u2010fold over suspensions), enhanced neuron (79.21\u00a0\u00b1\u00a06.67%\u00a0vs 65.08\u00a0\u00b1\u00a07.14%) and GABAergic neuron (29.85\u00a0\u00b1\u00a07.69%\u00a0vs 15.93\u00a0\u00b1\u00a010.33%) differentiation, and restored long\u2010term potentiation (LTP) to 97.89%\u00a0\u00b1\u00a019.84% of healthy control levels. Behavioral tests also show memory improvement, particularly in the Morris water maze. This 3D\u2010printed therapy not only holds potential for enhancing stem cell treatments but also addresses other 3D brain defects.<\/jats:p>","DOI":"10.1002\/advs.202510062","type":"journal-article","created":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T12:21:14Z","timestamp":1762518074000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Advanced Stem Cell Therapy: 3D\u2010Bioprinted Brain\u2010Like Transplants for Alzheimer's Disease\u2010Like Dementia"],"prefix":"10.1002","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9143-5290","authenticated-orcid":false,"given":"Ke","family":"Gai","sequence":"first","affiliation":[{"name":"Biomanufacturing Center Department of Mechanical Engineering Tsinghua University  Beijing 100084 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