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However, the rarity of CTCs in blood and their limited proliferation ability present significant challenges for establishing patient CTC\u2010derived xenograft (CDX) models. In this study, a novel 3D cellulose scaffold is developed for efficient isolation and in situ culture of CTCs, with the aim of constructing CDX models. Characterized by a macroporous and flexible structure, the 3D cellulose scaffold achieves high\u2010efficiency CTC capture while maintaining cell viability. Its excellent biocompatibility and permeability further promote the rapid and in situ growth of CTCs. Importantly, the self\u2010supporting and biodegradable scaffold allows for direct implantation into immunodeficient mice without leaving any residual material. This 3D cellulose scaffold has been successfully employed to construct CDX models from breast cancer patients. The application of this platform may enhance our understanding of metastasis mechanisms and offer significant benefits for cancer therapy.<\/jats:p>","DOI":"10.1002\/advs.202511148","type":"journal-article","created":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T11:49:10Z","timestamp":1773748150000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Capture and Culture of Circulating Tumor Cells Using a Biodegradable 3D Scaffold for Patient\u2010Derived Xenograft Construction"],"prefix":"10.1002","volume":"13","author":[{"given":"Yi\u2010Ke","family":"Wang","sequence":"first","affiliation":[{"name":"College of Chemistry and Molecular Sciences Wuhan University  Wuhan P. R. China"},{"name":"Henan Academy of Sciences Zhengzhou P. R. 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