{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T19:26:42Z","timestamp":1792006002501,"version":"4.4.0"},"reference-count":39,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2025,11,21]],"date-time":"2025-11-21T00:00:00Z","timestamp":1763683200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,11,21]],"date-time":"2025-11-21T00:00:00Z","timestamp":1763683200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32372610"],"award-info":[{"award-number":["32372610"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["U23A20201"],"award-info":[{"award-number":["U23A20201"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32160661"],"award-info":[{"award-number":["32160661"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32202359"],"award-info":[{"award-number":["32202359"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2022YFD1700300"],"award-info":[{"award-number":["2022YFD1700300"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["advanced.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Advanced Science"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The increasing prevalence of pesticide resistance in pathogenic bacteria, particularly among broad\u2010host\u2010range fungal pathogens such as\n                    <jats:italic>S. sclerotiorum<\/jats:italic>\n                    , poses a significant threat to global crop production and food security. Addressing this challenge requires the development of targeted compounds with novel mechanisms of action. Herein, a novel chromone\u2010acylhydrazone hybrid scaffold is designed and synthesized to specifically target fungal tubulin. Bioassay results identified compound G24 as a highly potent inhibitor of\n                    <jats:italic>S. sclerotiorum<\/jats:italic>\n                    (EC\n                    <jats:sub>50<\/jats:sub>\n                    = 0.21 \u00b5g\u00a0mL\n                    <jats:sup>\u22121<\/jats:sup>\n                    ), exhibiting superior efficacy compared to conventional fungicides. Mechanistic investigations, including molecular docking, molecular dynamics, and immunofluorescence staining, revealed that G24 effectively disrupts tubulin polymerization by forming hydrogen bonds with key tubulin residues. Notably, G24 exhibits selective antifungal activity while maintaining mammalian safety, addressing critical toxicity concerns. To enhance field performance, polyurethane microcapsules loaded with G24 (G24\u2010Loaded PU\u2010MCs) are developed with an encapsulation efficiency of 89.41%, facilitating slow\u2010release kinetics, improved foliar adhesion, and prolonged pathogen suppression. This integrated approach, combining targeted compound design with microencapsulation, offers a promising and sustainable strategy for combating pesticide resistance and promoting global food security.\n                  <\/jats:p>","DOI":"10.1002\/advs.202511492","type":"journal-article","created":{"date-parts":[[2025,11,22]],"date-time":"2025-11-22T03:40:37Z","timestamp":1763782837000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Novel Antifungal Scaffold Targeting Tubulin Overcomes\n                    <i>Sclerotinia Sclerotiorum<\/i>\n                    Resistance"],"prefix":"10.1002","volume":"13","author":[{"given":"Lihui","family":"Shao","sequence":"first","affiliation":[{"name":"State Key Laboratory of Green Pesticide Key Laboratory of Green Pesticide and Agricultural Bioengineering Ministry of Education Center for R&amp;D of Fine Chemicals of Guizhou University  Guiyang Guizhou 550025 P. R. China"},{"name":"School of Chemistry and Chemical Engineering Guizhou University  Guiyang Guizhou 550025 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xianqun","family":"Hu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Green Pesticide Key Laboratory of Green Pesticide and Agricultural Bioengineering Ministry of Education Center for R&amp;D of Fine Chemicals of Guizhou University  Guiyang Guizhou 550025 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Wu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Green Pesticide Key Laboratory of Green Pesticide and Agricultural Bioengineering Ministry of Education Center for R&amp;D of Fine Chemicals of Guizhou University  Guiyang Guizhou 550025 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiang","family":"Zhou","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Green Pesticide Key Laboratory of Green Pesticide and Agricultural Bioengineering Ministry of Education Center for R&amp;D of Fine Chemicals of Guizhou University  Guiyang Guizhou 550025 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0218-7641","authenticated-orcid":false,"given":"Bo","family":"Zhang","sequence":"additional","affiliation":[{"name":"Shanghai Engineering Research Center of Green Energy Chemical Engineering College of Chemistry and Materials Science Shanghai Normal University  100 Guilin Road Shanghai 200234 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Song","family":"Yang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Green Pesticide Key Laboratory of Green Pesticide and Agricultural Bioengineering Ministry of Education Center for R&amp;D of Fine Chemicals of Guizhou University  Guiyang Guizhou 550025 P. R. China"},{"name":"School of Chemistry and Chemical Engineering Guizhou University  Guiyang Guizhou 550025 P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,11,21]]},"reference":[{"key":"e_1_2_9_1_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-39562-7"},{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-024-48157-9"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2024.114588"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-024-49110-6"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fcr.2020.107803"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2021.113937"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.2c07264"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodcont.2022.108871"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-024-49484-7"},{"key":"e_1_2_9_10_1","volume":"24","author":"Liu X.","year":"2025","journal-title":"Adv. Sci."},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.5c04753"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2025.140226"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodhyd.2023.108697"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carbpol.2022.120260"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.0c06700"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2023.115711"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pestbp.2019.12.004"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41557-023-01239-5"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1080\/14756366.2020.1862100"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.3c04085"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioorg.2022.106069"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.1c04048"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jmedchem.1c00100"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmc.2017.09.043"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cej.2025.168956"},{"key":"e_1_2_9_26_1","volume":"525","author":"Jian X. W.","year":"2025","journal-title":"ACS Nano"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cej.2025.161401"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2024.137285"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cej.2024.157351"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.202403823"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.5b05119"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.indcrop.2018.05.037"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.2c06962"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioorg.2021.105239"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.1c08182"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carbpol.2022.119576"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cej.2022.137073"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cej.2024.151193"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.3c12352"}],"container-title":["Advanced Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/advs.202511492","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/advs.202511492","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/advs.202511492","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,17]],"date-time":"2026-02-17T09:30:04Z","timestamp":1771320604000},"score":1,"resource":{"primary":{"URL":"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/10.1002\/advs.202511492"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11,21]]},"references-count":39,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2026,2]]}},"alternative-id":["10.1002\/advs.202511492"],"URL":"https:\/\/doi.org\/10.1002\/advs.202511492","archive":["Portico"],"relation":{},"ISSN":["2198-3844","2198-3844"],"issn-type":[{"value":"2198-3844","type":"print"},{"value":"2198-3844","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,11,21]]},"assertion":[{"value":"2025-06-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-11-21","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e11492"}}