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However, viral diversity within wildlife remains insufficiently explored. This work performs an extensive analysis of 57\u00a0536 publicly high\u2010throughput sequencing datasets from wild mammals and birds, resulting in the generation of \u2248613.45 million assembled contigs, including 131\u00a0509 potential viral contigs identified through BLASTn and BLASTx searches. Following the exclusion of index hopping contamination, 9788 are categorized into 25 viral families with known zoonotic potential. These results indicate significant spatial and host\u2010specific variability in viral distribution and reveal a positive correlation between viral diversity and host biodiversity. Rodents, bats, ungulates, and anseriformes exhibit the highest viral diversity. Notably, 50% of the viral sequences exhibit &lt;90% amino acid identity to known viruses, indicating of potential novel viruses. Host\u2010virus network uncovers 458 associations, 67.9% are unreported. Further, sequences of avian influenza viruses are identified in goats, while SARS\u2010CoV\u20102 are detected in goats, ferrets, porpoises, cactus mice, and house finches. These findings highlight the largely uncharacterized viral diversity in wildlife, underscore the urgent requirement for surveillance at the wildlife\u2010livestock interfaces. Additionally, this work develop the Animal Pathogen Decoding Platform, to facilitate the retrieval and analysis of viral contigs, thereby reducing computational redundancies in future research.<\/jats:p>","DOI":"10.1002\/advs.202511920","type":"journal-article","created":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T10:52:55Z","timestamp":1758624775000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Unveiling Novel Viral Diversity, Biogeography, and Host Networks in Wildlife Through High\u2010Throughput Sequencing Data Mining"],"prefix":"10.1002","volume":"12","author":[{"given":"Hai","family":"Wang","sequence":"first","affiliation":[{"name":"School of Agriculture and Biology Shanghai Jiao Tong University  Shanghai 200240 China"},{"name":"State Key Laboratory for Animal Disease Control and Prevention Center for Emerging and Zoonotic Diseases College of Veterinary Medicine South China Agricultural University  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